RRC ID 71263
Author Shelomi M, Chen WM, Chen HK, Lee HY, Young CC, Lin SY, Liaw SJ.
Title Pseudomonas schmalbachii sp. nov., isolated from the gut of a millipede (Trigoniulus corallinus) from a coconut tree.
Journal Int J Syst Evol Microbiol
Abstract During an investigation of microbes associated with arthropods living in decaying coconut trees, a Pseudomonas isolate, Milli4T, was cultured from the digestive tract of the common Asian millipede, Trigoniulus corallinus. Sequence analysis of 16S rRNA and rpoB genes found that Milli4T was closely related but not identical to Pseudomonas panipatensis Esp-1T, Pseudomonas knackmussi B13T and Pseudomonas humi CCA1T. Whole genome sequencing suggested that this isolate represents a new species, with average nucleotide identity (OrthoANIu) values of around 83.9-87.7% with its closest relatives. Genome-to-genome distance calculations between Milli4T and its closest relatives also suggested they are distinct species. The genomic DNA G+C content of Milli4T was approximately 65.0 mol%. Phenotypic and chemotaxonomic characterization and fatty acid methyl ester analysis was performed on Milli4T and its related type strains. Based on these data, the new species Pseudomonas schmalbachii sp. nov. is proposed, and the type strain is Milli4T (=BCRC 81294T=JCM 34414T=CIP 111980T).
Volume 71(10)
Published 2021-10-1
DOI 10.1099/ijsem.0.005101
PMID 34705625
MeSH Animals Arthropods* / microbiology Bacterial Typing Techniques Base Composition Cocos DNA, Bacterial / genetics Fatty Acids / chemistry Nucleic Acid Hybridization Phylogeny* Pseudomonas / classification* Pseudomonas / isolation & purification RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
Resource
General Microbes JCM34414