論文 - 詳細
| RRC ID | 71368 |
|---|---|
| 著者 | Yu L, Zhou W, She Y, Ma H, Cai YS, Jiang M, Deng Z, Price NPJ, Chen W. |
| タイトル | Efficient biosynthesis of nucleoside cytokinin angustmycin A containing an unusual sugar system. |
| ジャーナル | Nat Commun |
| Abstract |
Angustmycin A has anti-mycobacterial and cytokinin activities, and contains an intriguing structure in which an unusual sugar with C5'-C6' dehydration is linked to adenine via an N-glycosidic bond. However, the logic underlying the biosynthesis of this molecule has long remained obscure. Here, we address angustmycin A biosynthesis by the full deciphering of its pathway. We demonstrate that AgmD, C, A, E, and B function as D-allulose 6-phosphate 3-epimerase, D-allulose 6-phosphate pyrophosphokinase, adenine phosphoallulosyltransferase, phosphoribohydrolase, and phosphatase, respectively, and that these collaboratively catalyze the relay reactions to biosynthesize angustmycin C. Additionally, we provide evidence that AgmF is a noncanonical dehydratase for the final step to angustmycin A via a self-sufficient strategy for cofactor recycling. Finally, we have reconstituted the entire six-enzyme pathway in vitro and in E. coli leading to angustmycin A production. These results expand the enzymatic repertoire regarding natural product biosynthesis, and also open the way for rational and rapid discovery of other angustmycin related antibiotics. |
| 巻・号 | 12(1) |
| ページ | 6633 |
| 公開日 | 2021-11-17 |
| DOI | 10.1038/s41467-021-26928-y |
| PII | 10.1038/s41467-021-26928-y |
| PMID | 34789759 |
| PMC | PMC8599513 |
| MeSH | Adenosine / analogs & derivatives* Adenosine / biosynthesis Adenosine / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism Biosynthetic Pathways Cytokinins / biosynthesis* Cytokinins / chemistry Dehydration Escherichia coli / genetics Escherichia coli / metabolism Fermentation Molecular Structure Multigene Family Nucleosides / biosynthesis* Nucleosides / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Streptomyces / enzymology Streptomyces / genetics |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM4053 |