論文 - 詳細
| RRC ID | 71426 |
|---|---|
| 著者 | Tominaga T. |
| タイトル | Rapid detection of total bacteria in foods using a poly-l-lysine-based lateral-flow assay. |
| ジャーナル | J Microbiol Methods |
| Abstract |
Food safety and freshness are evaluated according to microbiological load. To analyze this load rapidly, a poly-l-lysine-based lateral-flow assay (PLFA) was developed. A total of 90 strains of bacteria that are often detected in spoiled foods, including Enterobacteriaceae, lactic acid bacteria, Pseudomonas, and Bacillus were detected using the PLFA. A positive signal was obtained when the bacterial concentration was ≥6 log10 (cfu/test). A total of 36 fresh foods (meats, pastries, lettuces, cabbages, radishes, and sprouts) and corresponding spoiled foods were cultured for 0, 3, 6, and 9 h to investigate how many hours were required for microbial detection using PLFA. The higher the number of bacteria in a food, the shorter was the culture time required for PLFA-positive results to be obtained, so the distinction between fresh and spoiled food could be made based on the time taken for the culture to become PLFA-positive. The coefficient of determination of the least squares regression between the time to become PLFA-positive and the initial log10 (cfu/g) bacterial count for the food was 0.9888. The test time for the PLFA, including pretreatment, was approximately 15-30 min. This novel method will enable the detection of total bacteria on the food processing site. |
| 巻・号 | 183 |
| ページ | 106175 |
| 公開日 | 2021-4-1 |
| DOI | 10.1016/j.mimet.2021.106175 |
| PII | S0167-7012(21)00043-9 |
| PMID | 33640403 |
| MeSH | Bacteria / growth & development Bacteria / metabolism* Colony Count, Microbial Food Analysis / methods* Food Contamination / analysis* Food Handling Food Microbiology Lysine / metabolism* |
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| リソース情報 | |
| 一般微生物 | JCM1661 JCM1233 JCM1666 JCM1238 JCM9698 JCM11052 JCM2023 JCM6096 JCM7251 JCM1687 JCM1242 JCM1245 JCM12536 |