論文 - 詳細
| RRC ID | 71538 |
|---|---|
| 著者 | Okuma N, Soyano T, Suzaki T, Kawaguchi M. |
| タイトル | MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus. |
| ジャーナル | Nat Commun |
| Abstract |
Legumes utilize a shoot-mediated signaling system to maintain a mutualistic relationship with nitrogen-fixing bacteria in root nodules. In Lotus japonicus, shoot-to-root transfer of microRNA miR2111 that targets TOO MUCH LOVE, a nodulation suppressor in roots, has been proposed to explain the mechanism underlying nodulation control from shoots. However, the role of shoot-accumulating miR2111s for the systemic regulation of nodulation was not clearly shown. Here, we find L. japonicus has seven miR2111 loci, including those mapped through RNA-seq. MIR2111-5 expression in leaves is the highest among miR2111 loci and repressed after rhizobial infection depending on a shoot-acting HYPERNODULATION ABERRANT ROOT FORMATION1 (HAR1) receptor. MIR2111-5 knockout mutants show significantly decreased nodule numbers and miR2111 levels. Furthermore, grafting experiments using transformants demonstrate scions with altered miR2111 levels influence nodule numbers in rootstocks in a dose-dependent manner. Therefore, miR2111 accumulation in leaves through MIR2111-5 expression is required for HAR1-dependent systemic optimization of nodule number. |
| 巻・号 | 11(1) |
| ページ | 5192 |
| 公開日 | 2020-10-15 |
| DOI | 10.1038/s41467-020-19037-9 |
| PII | 10.1038/s41467-020-19037-9 |
| PMID | 33060582 |
| PMC | PMC7562733 |
| MeSH | Gene Expression Regulation, Plant Gene Knockout Techniques Lotus / genetics Lotus / metabolism* MicroRNAs / genetics MicroRNAs / metabolism* Plant Leaves Plant Proteins / genetics Plant Proteins / metabolism* Plant Root Nodulation / physiology* Plant Roots / metabolism Plant Shoots / genetics Plant Shoots / metabolism* Rhizobium / metabolism Root Nodules, Plant / growth & development* Sequence Analysis Signal Transduction / physiology Transcriptome |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | MG20 |