RRC ID 71602
著者 Kurogi T, Nabeshima K, Kataoka H, Okada Y, Koono M.
タイトル Stimulation of gelatinase B and tissue inhibitors of metalloproteinase (TIMP) production in co-culture of human osteosarcoma cells and human fibroblasts: gelatinase B production was stimulated via up-regulation of fibroblast growth factor (FGF) receptor.
ジャーナル Int J Cancer
Abstract Co-cultures of human osteosarcoma Takase (OST) cells with various human fibroblasts derived from surgical specimens stimulated production of gelatinase B (92-kDa type-IV collagenase, MMP-9), tissue inhibitors of metalloproteinase (TIMP)-1, and TIMP-2 when compared to cultures of individual cells. The maximum stimulation of gelatinase-B production occurred at a cellular ratio of 1:1. Conditioned media from several fibroblast cultures stimulated OST cells to produce gelatinase B, TIMP-1 and TIMP-2, but not vice versa. Among various recombinant growth factors or cytokines, tumor necrosis factor (TNF)-alpha stimulated gelatinase-B production in cultures of OST cells alone, while recombinant basic fibroblast growth factor (bFGF) stimulated gelatinase-B production in co-cultures of OST cells with skin fibroblasts but not in individual cultures of each cell type. In the co-cultures, gelatinase-B production was inhibited by anti-bFGF monoclonal antibody (MAb), but not by anti TNF-alpha MAb. This co-culture-specific stimulation of gelatinase-B production by bFGF was associated with increased expression of the FGF receptor in the co-culture.
巻・号 66(1)
ページ 82-90
公開日 1996-3-28
DOI 10.1002/(SICI)1097-0215(19960328)66:1<82::AID-IJC15>3.0.CO;2-D
PII 10.1002/(SICI)1097-0215(19960328)66:1<82::AID-IJC15>3.0.CO;2-D
PMID 8608972
MeSH Animals Cells, Cultured Collagenases / metabolism* Culture Media Cytokines / metabolism Fibroblast Growth Factor 2 / metabolism Fibroblasts / enzymology* Glycoproteins / metabolism* Growth Substances / metabolism Humans Matrix Metalloproteinase 9 Mice Osteosarcoma / enzymology* Receptors, Fibroblast Growth Factor / metabolism Tissue Inhibitor of Metalloproteinases Up-Regulation
IF 5.145
リソース情報
ヒト・動物細胞 OST(RCB0454)