RRC ID |
71602
|
著者 |
Kurogi T, Nabeshima K, Kataoka H, Okada Y, Koono M.
|
タイトル |
Stimulation of gelatinase B and tissue inhibitors of metalloproteinase (TIMP) production in co-culture of human osteosarcoma cells and human fibroblasts: gelatinase B production was stimulated via up-regulation of fibroblast growth factor (FGF) receptor.
|
ジャーナル |
Int J Cancer
|
Abstract |
Co-cultures of human osteosarcoma Takase (OST) cells with various human fibroblasts derived from surgical specimens stimulated production of gelatinase B (92-kDa type-IV collagenase, MMP-9), tissue inhibitors of metalloproteinase (TIMP)-1, and TIMP-2 when compared to cultures of individual cells. The maximum stimulation of gelatinase-B production occurred at a cellular ratio of 1:1. Conditioned media from several fibroblast cultures stimulated OST cells to produce gelatinase B, TIMP-1 and TIMP-2, but not vice versa. Among various recombinant growth factors or cytokines, tumor necrosis factor (TNF)-alpha stimulated gelatinase-B production in cultures of OST cells alone, while recombinant basic fibroblast growth factor (bFGF) stimulated gelatinase-B production in co-cultures of OST cells with skin fibroblasts but not in individual cultures of each cell type. In the co-cultures, gelatinase-B production was inhibited by anti-bFGF monoclonal antibody (MAb), but not by anti TNF-alpha MAb. This co-culture-specific stimulation of gelatinase-B production by bFGF was associated with increased expression of the FGF receptor in the co-culture.
|
巻・号 |
66(1)
|
ページ |
82-90
|
公開日 |
1996-3-28
|
DOI |
10.1002/(SICI)1097-0215(19960328)66:1<82::AID-IJC15>3.0.CO;2-D
|
PII |
10.1002/(SICI)1097-0215(19960328)66:1<82::AID-IJC15>3.0.CO;2-D
|
PMID |
8608972
|
MeSH |
Animals
Cells, Cultured
Collagenases / metabolism*
Culture Media
Cytokines / metabolism
Fibroblast Growth Factor 2 / metabolism
Fibroblasts / enzymology*
Glycoproteins / metabolism*
Growth Substances / metabolism
Humans
Matrix Metalloproteinase 9
Mice
Osteosarcoma / enzymology*
Receptors, Fibroblast Growth Factor / metabolism
Tissue Inhibitor of Metalloproteinases
Up-Regulation
|
IF |
5.145
|
リソース情報 |
ヒト・動物細胞 |
OST(RCB0454) |