論文 - 詳細
| RRC ID | 7167 |
|---|---|
| 著者 | Kupinski AP, Müller-Reichert T, Eckmann CR. |
| タイトル | The Caenorhabditis elegans Ste20 kinase, GCK-3, is essential for postembryonic developmental timing and regulates meiotic chromosome segregation. |
| ジャーナル | Dev Biol |
| Abstract |
Ste20 kinases constitute a large family of serine/threonine kinases with a plethora of biological functions. Members of the GCK-VI subfamily have been identified as important regulators of osmohomeostasis across species functioning upstream of ion channels. Although the expression of the two highly similar mammalian GCK-VI kinases is eminent in a wide variety of tissues, which includes also the testis, their potential roles in development remain elusive. Caenorhabditis elegans contains a single ancestral ortholog termed GCK-3. Here, we report a comprehensive analysis of gck-3 function and demonstrate its requirement for several developmental processes independent of ion homeostasis, i.e., larval progression, vulva, and germ line formation. Consistent with a wide range of gck-3 function we find that endogenous GCK-3 is expressed ubiquitously. The serine/threonine kinase activity of GCK-3, but not its presumed C-terminal substrate interaction domain, is essential for gck-3 gene function. Although expressed in female germ cells, we find GCK-3 progressively accumulating during spermatogenesis where it promotes the first meiotic cell division and facilitates faithful chromosome segregation. In particular, we find that different levels of gck-3 activity appear to be important for various aspects of germ line development. Taken together, our findings suggest that members of the GCK-VI kinase subfamily may act as key regulators of many developmental processes and that this newly described role in meiotic progression might be conserved and an important part of sexual reproduction. |
| 巻・号 | 344(2) |
| ページ | 758-71 |
| 公開日 | 2010-8-15 |
| DOI | 10.1016/j.ydbio.2010.05.505 |
| PII | S0012-1606(10)00808-0 |
| PMID | 20595048 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Cell Division Chromosome Segregation Female Genes Germ Cells / metabolism Male Meiosis Phosphotransferases / genetics Phosphotransferases / metabolism Protein Serine-Threonine Kinases / chemistry Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism* Vulva / metabolism |
| IF | 2.896 |
| 引用数 | 11 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1296 |