RRC ID 7167
Author Kupinski AP, Müller-Reichert T, Eckmann CR.
Title The Caenorhabditis elegans Ste20 kinase, GCK-3, is essential for postembryonic developmental timing and regulates meiotic chromosome segregation.
Journal Dev. Biol.
Abstract Ste20 kinases constitute a large family of serine/threonine kinases with a plethora of biological functions. Members of the GCK-VI subfamily have been identified as important regulators of osmohomeostasis across species functioning upstream of ion channels. Although the expression of the two highly similar mammalian GCK-VI kinases is eminent in a wide variety of tissues, which includes also the testis, their potential roles in development remain elusive. Caenorhabditis elegans contains a single ancestral ortholog termed GCK-3. Here, we report a comprehensive analysis of gck-3 function and demonstrate its requirement for several developmental processes independent of ion homeostasis, i.e., larval progression, vulva, and germ line formation. Consistent with a wide range of gck-3 function we find that endogenous GCK-3 is expressed ubiquitously. The serine/threonine kinase activity of GCK-3, but not its presumed C-terminal substrate interaction domain, is essential for gck-3 gene function. Although expressed in female germ cells, we find GCK-3 progressively accumulating during spermatogenesis where it promotes the first meiotic cell division and facilitates faithful chromosome segregation. In particular, we find that different levels of gck-3 activity appear to be important for various aspects of germ line development. Taken together, our findings suggest that members of the GCK-VI kinase subfamily may act as key regulators of many developmental processes and that this newly described role in meiotic progression might be conserved and an important part of sexual reproduction.
Volume 344(2)
Pages 758-71
Published 2010-8-15
DOI 10.1016/j.ydbio.2010.05.505
PII S0012-1606(10)00808-0
PMID 20595048
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Cell Division Chromosome Segregation Female Genes Germ Cells / metabolism Male Meiosis Phosphotransferases / genetics Phosphotransferases / metabolism Protein-Serine-Threonine Kinases / chemistry Protein-Serine-Threonine Kinases / genetics* Protein-Serine-Threonine Kinases / metabolism* Vulva / metabolism
IF 2.936
Times Cited 8
WOS Category DEVELOPMENTAL BIOLOGY
Resource
C.elegans tm1296