論文 - 詳細
| RRC ID | 71718 |
|---|---|
| 著者 | Iida S, Akiyama Y, Nakajima T, Ichikawa W, Nihei Z, Sugihara K, Yuasa Y. |
| タイトル | Alterations and hypermethylation of the p14(ARF) gene in gastric cancer. |
| ジャーナル | Int J Cancer |
| Abstract |
p14(ARF), generated through an alternative splicing process that replaces the first exon, 1alpha, of p16(INK4a) with exon 1beta, located >15 kb upstream of exon 1alpha, has been shown to function as a growth suppressor. We examined 11 gastric cancer cell lines for mRNA expression, homozygous deletion, mutation, and promoter methylation of the p14(ARF) gene. No mRNA expression was detected in 5 of the 7 diffuse-type cell lines. All intestinal cell lines displayed normal levels of expression except for one with a low level of expression. Of the 5 cell lines without expression, 3 (MKN45, NUGC-2, and NUGC-4) and 1 (KATO III) displayed homozygous deletion and methylation of the p14(ARF) gene, respectively. No mutation was found in the whole coding region of the p14(ARF) gene in 8 cell lines without homozygous deletion. Our results indicate that the p14(ARF) gene is more frequently inactivated by homozygous deletion or methylation in diffuse-type gastric cancer cell lines (5/7, 71.4%) than in intestinal ones (0/4, P = 0.022). When we also analyzed 62 primary gastric cancers for the methylation status of the p14(ARF) promoter region, the methylation frequency tended to be higher in diffuse-type gastric cancers (15/33, 45.5%) than in intestinal ones (7/28, 25%). Thus, p14(ARF) alterations might be involved in diffuse-type gastric carcinogenesis. |
| 巻・号 | 87(5) |
| ページ | 654-8 |
| 公開日 | 2000-9-1 |
| DOI | 10.1002/1097-0215(20000901)87:5<654::aid-ijc6>3.0.co;2-p |
| PII | 10.1002/1097-0215(20000901)87:5<654::AID-IJC6>3.0.CO;2-P |
| PMID | 10925358 |
| MeSH | Carrier Proteins / genetics Cyclin-Dependent Kinase Inhibitor p16 DNA Methylation* Gene Deletion Gene Expression Homozygote Humans Immunohistochemistry Mutation Promoter Regions, Genetic / physiology Protein Biosynthesis Proteins / genetics* RNA, Messenger / biosynthesis RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms / genetics* Tumor Cells, Cultured Tumor Suppressor Protein p14ARF Tumor Suppressor Protein p53 / biosynthesis Tumor Suppressor Protein p53 / genetics |
| IF | 5.145 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | GCIY(RCB0555) TGBC11TKB(RCB1148) |