RRC ID 7178
Author Mizuta Y, Harushima Y, Kurata N.
Title Rice pollen hybrid incompatibility caused by reciprocal gene loss of duplicated genes.
Journal Proc Natl Acad Sci U S A
Abstract Genetic incompatibility is a barrier contributing to species isolation and is caused by genetic interactions. We made a whole genome survey of two-way interacting loci acting within the gametophyte or zygote using independence tests of marker segregations in an F(2) population from an intersubspecific cross between O. sativa subspecies indica and japonica. We detected only one reproducible interaction, and identified paralogous hybrid incompatibility genes, DOPPELGANGER1 (DPL1) and DOPPELGANGER2 (DPL2), by positional cloning. Independent disruptions of DPL1 and DPL2 occurred in indica and japonica, respectively. DPLs encode highly conserved, plant-specific small proteins (∼10 kDa) and are highly expressed in mature anther. Pollen carrying two defective DPL alleles became nonfunctional and did not germinate, suggesting an essential role for DPLs in pollen germination. Although rice has many duplicated genes resulting from ancient whole genome duplication, the origin of this gene duplication was in recent small-scale gene duplication, occurring after Oryza-Brachypodium differentiation. Comparative analyses suggested the geographic and phylogenetic distribution of these two defective alleles, showing that loss-of-function mutations of DPL1 genes emerged multiple times in indica and its wild ancestor, O. rufipogon, and that the DPL2 gene defect is specific to japonica cultivars.
Volume 107(47)
Pages 20417-22
Published 2010-11-23
DOI 10.1073/pnas.1003124107
PII 1003124107
PMID 21048083
PMC PMC2996679
MeSH Base Sequence Chromosome Mapping Cloning, Molecular DNA, Complementary / genetics Gene Components Gene Frequency Genes, Duplicate / genetics* Genes, Plant / genetics* Genetic Complementation Test Genetic Speciation* Genomics / methods Germination / genetics Hybridization, Genetic* Immunoblotting In Situ Hybridization Molecular Sequence Data Mutation / genetics Oryza / genetics* Pollen / genetics* Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA
IF 9.412
Times Cited 95
Rice W1958 W1945 W0120 W0108 W0106 W1681 W1866 W0610 W2003 W1294 W1921 W1236 W1588 W0698 W1169=W1165 W1187 W1508 W1540 W1625 W1635 W0002 T0421 T0710 T0724 C0025 C8863