RRC ID 7180
Author Saito T, Ariizumi T, Okabe Y, Asamizu E, Hiwasa-Tanase K, Fukuda N, Mizoguchi T, Yamazaki Y, Aoki K, Ezura H.
Title TOMATOMA: a novel tomato mutant database distributing Micro-Tom mutant collections.
Journal Plant Cell Physiol.
Abstract The tomato is an excellent model for studies of plants bearing berry-type fruits and for experimental studies of the Solanaceae family of plants due to its conserved genetic organization. In this study, a comprehensive mutant tomato population was generated in the background of Micro-Tom, a dwarf, rapid-growth variety. In this and previous studies, a family including 8,598 and 6,422 M(2) mutagenized lines was produced by ethylmethane sulfonate (EMS) mutagenesis and γ-ray irradiation, and this study developed and investigated these M(2) plants for alteration of visible phenotypes. A total of 9,183 independent M(2) families comprising 91,830 M(2) plants were inspected for phenotypic alteration, and 1,048 individual mutants were isolated. Subsequently, the observed mutant phenotypes were classified into 15 major categories and 48 subcategories. Overall, 1,819 phenotypic categories were found in 1,048 mutants. Of these mutants, 549 were pleiotropic, whereas 499 were non-pleiotropic. Multiple different mutant alleles per locus were found in the mutant libraries, suggesting that the mutagenized populations were nearly saturated. Additionally, genetic analysis of backcrosses indicated the successful inheritance of the mutations in BC(1)F(2) populations, confirming the reproducibility in the morphological phenotyping of the M(2) plants. To integrate and manage the visible phenotypes of mutants and other associated data, we developed the in silico database TOMATOMA, a relational system interfacing modules between mutant line names and phenotypic categories. TOMATOMA is a freely accessible database, and these mutant recourses are available through the TOMATOMA (http://tomatoma.nbrp.jp/index.jsp).
Volume 52(2)
Pages 283-96
Published 2011-2
DOI 10.1093/pcp/pcr004
PII pcr004
PMID 21258066
PMC PMC3037083
MeSH Alleles Crosses, Genetic DNA, Plant / genetics Databases, Genetic* Gene Library Lycopersicon esculentum / genetics* Mutation Phenotype* Sequence Analysis, DNA
IF 4.76
Times Cited 64
WOS Category PLANT SCIENCES CELL BIOLOGY
Resource
Tomato TOMJPE2811-1 TOMJPE5405-1 TOMJPE5405-2 TOMJPG0114-1 TOMJPG0226-1 TOMJPG3699 TOMJPE6000-1 TOMJPG5663-1 TOMJPE6152-1 TOMJPG4290 TOMJPE5414-2 TOMJPG2941-1 TOMJPE1832-1 TOMJPG1331-1 TOMJPG2614 TOMJPE6398-1 TOMJPE6352 TOMJPE6472-1 TOMJPE5236-1 TOMJPE6397 TOMJPE5278 TOMJPE8506 TOMJPG1450-1 TOMJPG2156-1 TOMJPE6371-1 TOMJPE6558-1 TOMJPE6574-2 TOMJPE6360-1 TOMJPE8910-1 TOMJPE8534-1 TOMJPG1949-1 TOMJPG1700-1 TOMJPG2075-1 TOMJPE8505-1 TOMJPG2082-1 TOMJPG1572-1 TOMJPG3104-1 TOMJPE8533-1 TOMJPE6517-1 TOMJPG1890-1 TOMJPG2385-1 TOMJPE6646-1 TOMJPE8949-1 TOMJPE6574-1 TOMJPG1366-1 TOMJPG2574-1 TOMJPE3139-1? TOMJPG0319-1? TOMJPG2702-1 TOMJPE5044-1 TOMJPG4497-1 TOMJPG2637-1 TOMJPF00001 TOMJPF00002(Moneymaker) TOMJPF00003(Aichi-first) TOMJPF00004(Ailsa Craig) TOMJPF00005(M82) TOMJPF00006(Rutgers) TOMJPF00007(Ponderosa) TOMJPF00008(pennellii) TOMJPF00009-1(Cerasiforme) TOMJPF00010-1(peruvianum) TOMJPF00011-1(chilense) TOMJPF00012-1(pimpinellifolium) TOMJPF00013(Introgression lines) TOMJPF00014(habrochaites) TOMATOMA database