RRC ID 71854
Author Nagawa S, Sawa S, Sato S, Kato T, Tabata S, Fukuda H.
Title Gene trapping in Arabidopsis reveals genes involved in vascular development.
Journal Plant Cell Physiol
Abstract The procambium is made up of stem cells that give rise to various vascular cells in plants. To understand the molecular nature of procambium cells, we tried to identify genes that characterize procambium cells using Arabidopsis gene trap lines. Among 26,000 gene trap lines, we found 67 lines in which beta-glucuronidase (GUS) staining occurred along vascular tissues in cotyledons and/or adult leaves. Although four gene trap lines showed procambium-preferential GUS expression, their expression patterns differed from each other during procambium development in root tips and young rosette leaves. Genomic regions flanking the gene trap insertion points in 25 of the 67 lines were determined, including three lines showing preferential GUS staining of the procambium. The three procambium-related genes encoded PINHEAD, katanin and an unknown DUF740 domain-containing protein. We discuss procambium development based on the functions and the differential GUS staining patterns of the procambium-related genes.
Volume 47(10)
Pages 1394-405
Published 2006-10-1
DOI 10.1093/pcp/pcl009
PII pcl009
PMID 16980704
MeSH Adenosine Triphosphatases / analysis Adenosine Triphosphatases / genetics Arabidopsis / genetics* Arabidopsis Proteins / analysis Arabidopsis Proteins / genetics Cotyledon / genetics Gene Expression Profiling Gene Expression Regulation, Plant* Genome, Plant* Glucuronidase / analysis Glucuronidase / genetics Katanin Meristem / genetics Phloem / genetics Plant Leaves / genetics Staining and Labeling Xylem / genetics
IF 4.062
Arabidopsis / Cultured plant cells, genes rpd00105