論文 - 詳細
| RRC ID | 71861 |
|---|---|
| 著者 | Yamashiro C, Tokuda K, Kobayashi Y, Higashijima F, Yoshimoto T, Ota M, Ogata T, Ashimori A, Kobayashi M, Hatano M, Uchi SH, Wakuta M, Teranishi S, Kimura K. |
| タイトル | Benzalkonium chloride-induced myofibroblastic transdifferentiation of Tenon's capsule fibroblasts is inhibited by coculture with corneal epithelial cells or by interleukin-10. |
| ジャーナル | Sci Rep |
| Abstract |
Benzalkonium chloride (BAC) is used as a preservative in eyedrops but induces subconjunctival fibrosis that can result in failure of glaucoma surgery. Tenon's capsule fibroblasts in subconjunctival tissue interact with the corneal epithelium through tear fluid. With the use of a coculture system, we have now investigated the effect of human corneal epithelial (HCE) cells on myofibroblastic transdifferentiation of human Tenon fibroblasts (HTFs) induced by BAC (5 × 10-6%). Immunofluorescence and immunoblot analyses revealed that the BAC-induced expression of α smooth muscle actin (αSMA) in HTFs was suppressed by coculture of these cells with HCE cells (p < 0.01). The concentration of interleukin-10 (IL-10) in culture supernatants of BAC-treated HTFs was increased by coculture with HCE cells (17.26-fold, vs. coculure, p < 0.001). Immunofluorescence and immunoblot analyses also showed that exogenous IL-10 (300 pg/ml) suppressed the BAC-induced expression of αSMA by 43.65% (p < 0.05) as well as the nuclear translocation of myocardin-related transcription factor-A (MRTF-A) by 39.32% (p < 0.01) in HTFs cultured alone. Our findings suggest that corneal epithelial cells may protect against subconjunctival fibrosis by maintaining IL-10 levels and preventing the MRTF-A-dependent transdifferentiation of HTFs into myofibroblasts. |
| 巻・号 | 11(1) |
| ページ | 16096 |
| 公開日 | 2021-8-9 |
| DOI | 10.1038/s41598-021-94852-8 |
| PII | 10.1038/s41598-021-94852-8 |
| PMID | 34373467 |
| PMC | PMC8352883 |
| MeSH | Actins / metabolism Benzalkonium Compounds / pharmacology* Cell Proliferation / drug effects Cell Transdifferentiation / drug effects* Cells, Cultured Coculture Techniques / methods Cornea / drug effects* Cornea / metabolism Epithelial Cells / drug effects* Epithelial Cells / metabolism Fibroblasts / drug effects* Fibroblasts / metabolism Fibrosis / drug therapy Fibrosis / metabolism Humans Interleukin-10 / metabolism* Myofibroblasts / drug effects* Myofibroblasts / metabolism Signal Transduction / drug effects Tenon Capsule / drug effects* Tenon Capsule / metabolism Trans-Activators / metabolism |
| IF | 3.998 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |