RRC ID 719
Author Nitasaka E.
Title Insertion of an En/Spm-related transposable element into a floral homeotic gene DUPLICATED causes a double flower phenotype in the Japanese morning glory.
Journal Plant J.
Abstract Mutations in a floral homeotic gene DUPLICATED (DP) in the Japanese morning glory (Ipomoea nil) cause a substitution of reproductive organs to perianth organs (petals and sepals). This phenotype is similar to loss-of-function phenotypes of the C-function MCM1, AGAMOUS, DEFICIENS and SRF (MADS)-box gene family of transcription factors. DP was isolated using the consensus sequence of C-function MADS-box genes. Its intron-exon structure was well conserved beyond species, and it belongs to the FARINELLI (FAR)-pMADS3 subclass of C-function MADS-box genes. In a dp mutant, an Enhancer/Suppressor-mutator (En/Spm)-related transposable element, transposable element of Pharbitis nil (Tpn)-botan, was inserted in the second intron of DP gene, and the subsequent excision event led to a deletion of a substantial part of the original Tpn element and DP genome. A segment of Tpn1-related transposable element was identified at the recent insertion site of the wild-type DP genome. This transposable element sequence was present in all Ipomoea species tested. This finding suggests that the insertion event originated in an ancestral species of genus Ipomoea.
Volume 36(4)
Pages 522-31
Published 2003-11
PII 1896
PMID 14617082
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular DNA Transposable Elements / genetics* DNA, Complementary / chemistry DNA, Complementary / genetics DNA, Plant / chemistry DNA, Plant / genetics Flowers / genetics* Genotype Ipomoea / genetics* MADS Domain Proteins / genetics MADS Domain Proteins / metabolism Molecular Sequence Data Mutagenesis, Insertional Mutation Phenotype Phylogeny Plant Proteins / genetics* Plant Proteins / metabolism Sequence Analysis, DNA Sequence Homology, Nucleic Acid
IF 5.726
Times Cited 47
Morning Glory AK1