| RRC ID |
7197
|
| 著者 |
Mizuno D, Agata H, Furue H, Kimura A, Narita Y, Watanabe N, Ishii Y, Ueda M, Tojo A, Kagami H.
|
| タイトル |
Limited but heterogeneous osteogenic response of human bone marrow mesenchymal stem cells to bone morphogenetic protein-2 and serum.
|
| ジャーナル |
Growth Factors
|
| Abstract |
Although there are numerous reports describing the in vivo bone forming capability of recombinant human bone morphogenetic proteins-2 (rhBMP-2), studies have reported limited effects on human mesenchymal stem cells (hMSCs). However, the reasons for these discrepancies are not well understood. The aim of this study was to investigate the responsiveness of hMSCs to osteoinductive signals, focusing on rhBMP-2 and the effect of serum on that responsiveness. Human MSCs from six donors were analysed. When those cells were treated with osteoinduction medium including dexamethasone (Dex), alkaline phosphatase (ALP) activities increased in all cell lines. On the other hand, rhBMP-2-containing medium failed to increase ALP activity. When five different sera were used for cultivation and induction with rhBMP-2, ALP activities increased in two of them, but not in the others. The expression of BMP-2 antagonist noggin was induced in almost all combinations regardless of the responsiveness to rhBMP-2. On the other hand, the expression of follistatin showed significant variations depending on the serum and cell line. However, the expression did not correlate with the responsiveness to rhBMP-2. The results from this study showed limited but heterogeneous osteogenic response of hMSCs to rhBMP-2 and that the results are affected by the choice of serum. This fact should be concerned for the successful and effective clinical application of rhBMP-2.
|
| 巻・号 |
28(1)
|
| ページ |
34-43
|
| 公開日 |
2010-2-1
|
| DOI |
10.3109/08977190903326362
|
| PII |
10.3109/08977190903326362
|
| PMID |
19835486
|
| MeSH |
Adult
Alkaline Phosphatase / metabolism
Animals
Bone Marrow Cells / physiology*
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / metabolism
Bone Morphogenetic Proteins / pharmacology*
Cell Differentiation
Cells, Cultured
Dexamethasone / pharmacology
Female
Femur / drug effects
Humans
Male
Mesenchymal Stem Cells* / cytology
Mesenchymal Stem Cells* / drug effects
Mice
Mice, Inbred BALB C
Osteoblasts / physiology
Osteogenesis / drug effects*
Recombinant Proteins / metabolism
Recombinant Proteins / pharmacology*
Serum / physiology*
Transforming Growth Factor beta / metabolism
Transforming Growth Factor beta / pharmacology*
Young Adult
|
| IF |
1.543
|
| 引用数 |
18
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MSC-R53-1(HMS4900) |
