RRC ID 7221
Author Krapp A, Del Rosario EC, Simanis V.
Title The role of Schizosaccharomyces pombe dma1 in spore formation during meiosis.
Journal J. Cell. Sci.
Abstract Meiosis is a specialised form of the cell cycle that gives rise to haploid gametes. In Schizosaccharomyces pombe, the products of meiosis are four spores, which are formed by encapsulation of the four meiosis II nuclei within the cytoplasm of the zygote produced by fusion of the mating cells. The S. pombe spindle pole body is remodelled during meiosis II and membrane vesicles are then recruited there to form the forespore membrane, which encapsulates the haploid nucleus to form a prespore. Spore wall material is then deposited, giving rise to the mature spore. The septation initiation network is required to coordinate cytokinesis and mitosis in the vegetative cycle and for spore formation in the meiotic cycle. We have investigated the role of the SIN regulator dma1p in meiosis; we find that although both meiotic divisions occur in the absence of dma1p, asci frequently contain fewer than four spores, which are larger than in wild-type meiosis. Our data indicate that dma1p acts in parallel to the leading-edge proteins and septins to assure proper formation for the forespore membrane. Dma1p also contributes to the temporal regulation of the abundance of the meiosis-specific SIN component mug27p.
Volume 123(Pt 19)
Pages 3284-93
Published 2010-10-1
DOI 10.1242/jcs.069112
PII jcs.069112
PMID 20826461
MeSH Animals Animals, Genetically Modified Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Colony Count, Microbial Meiosis / genetics Membrane Fusion / genetics Mutant Proteins / genetics Mutant Proteins / metabolism* Protein Kinases / metabolism* Protein Transport / genetics Protein-Serine-Threonine Kinases / metabolism* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Signal Transduction Spores, Fungal / genetics Spores, Fungal / isolation & purification Transgenes / genetics
IF 4.431
Times Cited 11
WOS Category CELL BIOLOGY
Resource
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