RRC ID 72374
Author Ando Y, Kuroda M, Honda O, Asaumi J, Okumura Y, Takeda Y, Akaki S, Kanazawa S, Kawasaki S, Hiraki Y.
Title The effect of calcium on Fas-mediated apoptosis and secondary necrosis of Jurkat cells.
Journal Int J Mol Med
Abstract Our previous study using a new microscopic system indicated that apoptotic cells undergo secondary necrosis during treatment with anti-Fas antibody and calcium. In this study, we compared the time-lapse appearance of apoptosis and secondary necrosis of Jurkat cells during treatment with anti-Fas antibody with and without calcium in individual cells. Apoptosis developed in 97% and 81% of cells during 36-hour-treatment with anti-Fas antibody with and without calcium, respectively. The apoptosis of Jurkat cells showed a characteristic pattern of time-lapse morphological change. Less than 1% of the apoptotic cells divided into apoptotic bodies. There was budding in all the other apoptotic cells, but no apoptotic bodies formed. We confirmed that secondary necrosis occurs in individual apoptotic cells during treatment with anti-Fas antibody. Neither the pattern of time-lapse morphological change nor the time interval between the beginning of apoptotic budding and secondary necrosis were related to the presence of calcium. This study clarified the characteristic pattern of time-lapse morphological change in Jurkat cells during treatment with anti-Fas antibody, and presented direct evidence that individual apoptotic cells undergo secondary necrosis. The presence of calcium did not affect the pattern of morphological change or the time interval between the beginning of apoptotic budding and secondary necrosis.
Volume 7(3)
Pages 243-7
Published 2001-3-1
DOI 10.3892/ijmm.7.3.243
PMID 11179501
MeSH Apoptosis* Calcium / metabolism* Cell Size* DNA Fragmentation Humans Immunohistochemistry Jurkat Cells Necrosis* Time Factors fas Receptor / metabolism*
Resource
Human and Animal Cells Jurkat(RCB0806)