RRC ID 72418
Author Nakamura K, Funakoshi H, Miyamoto K, Tokunaga F, Nakamura T.
Title Molecular cloning and functional characterization of a human scavenger receptor with C-type lectin (SRCL), a novel member of a scavenger receptor family.
Journal Biochem Biophys Res Commun
Abstract Using a human placenta cDNA library, we cloned a novel member belonging to the scavenger receptor family. Complementary DNA of this clone encodes a type II transmembranous glycoprotein containing a collagen-like domain, which are typical structural characteristics of scavenger receptor class A. This protein also contains a C-type lectin/carbohydrate recognition domain (C-type CRD) located at the C-terminus. We designated this as Scavenger Receptor with C-type Lectin (SRCL) type I. We also isolated human SRCL type II, which lacks the C-type CRD. Northern blot analysis revealed that hSRCL type I and type II mRNAs are abundantly expressed in adult human tissues. When hSRCL type I and type II were expressed in CHO-K1 cells, they were localized in the plasma membrane forming clusters on the surface. Ligand-binding studies of CHO-K1 cells expressing hSRCL type I and type II demonstrated their specific binding capacity in Escherichia coli and Staphylococcus aureus. These results indicate that hSRCL is a novel bacteria-binding receptor containing a C-type CRD and this receptor may play an important role in host defense.
Volume 280(4)
Pages 1028-35
Published 2001-2-2
DOI 10.1006/bbrc.2000.4210
PII S0006-291X(00)94210-4
PMID 11162630
MeSH Amino Acid Sequence Animals Base Sequence Blotting, Northern CHO Cells Cell Membrane / metabolism Chromosome Mapping Chromosomes, Human, Pair 18 Cloning, Molecular Collagen / chemistry Collectins* Cricetinae DNA, Complementary / metabolism Escherichia coli / metabolism Gene Library Glycoproteins / chemistry Humans Immunohistochemistry Ligands Membrane Proteins* Microscopy, Fluorescence Models, Biological Molecular Sequence Data Placenta / metabolism Precipitin Tests Protein Binding Protein Structure, Tertiary Receptors, Immunologic / biosynthesis Receptors, Immunologic / chemistry* Receptors, Immunologic / genetics* Receptors, Lipoprotein* Receptors, Scavenger Scavenger Receptors, Class A Scavenger Receptors, Class B Sequence Homology, Amino Acid Staphylococcus aureus / metabolism Tissue Distribution
IF 2.985
Human and Animal Cells CHO-K1(RCB0285)