| RRC ID |
72418
|
| 著者 |
Nakamura K, Funakoshi H, Miyamoto K, Tokunaga F, Nakamura T.
|
| タイトル |
Molecular cloning and functional characterization of a human scavenger receptor with C-type lectin (SRCL), a novel member of a scavenger receptor family.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Using a human placenta cDNA library, we cloned a novel member belonging to the scavenger receptor family. Complementary DNA of this clone encodes a type II transmembranous glycoprotein containing a collagen-like domain, which are typical structural characteristics of scavenger receptor class A. This protein also contains a C-type lectin/carbohydrate recognition domain (C-type CRD) located at the C-terminus. We designated this as Scavenger Receptor with C-type Lectin (SRCL) type I. We also isolated human SRCL type II, which lacks the C-type CRD. Northern blot analysis revealed that hSRCL type I and type II mRNAs are abundantly expressed in adult human tissues. When hSRCL type I and type II were expressed in CHO-K1 cells, they were localized in the plasma membrane forming clusters on the surface. Ligand-binding studies of CHO-K1 cells expressing hSRCL type I and type II demonstrated their specific binding capacity in Escherichia coli and Staphylococcus aureus. These results indicate that hSRCL is a novel bacteria-binding receptor containing a C-type CRD and this receptor may play an important role in host defense.
|
| 巻・号 |
280(4)
|
| ページ |
1028-35
|
| 公開日 |
2001-2-2
|
| DOI |
10.1006/bbrc.2000.4210
|
| PII |
S0006-291X(00)94210-4
|
| PMID |
11162630
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
CHO Cells
Cell Membrane / metabolism
Chromosome Mapping
Chromosomes, Human, Pair 18
Cloning, Molecular
Collagen / chemistry
Collectins*
Cricetinae
DNA, Complementary / metabolism
Escherichia coli / metabolism
Gene Library
Glycoproteins / chemistry
Humans
Immunohistochemistry
Ligands
Membrane Proteins*
Microscopy, Fluorescence
Models, Biological
Molecular Sequence Data
Placenta / metabolism
Precipitin Tests
Protein Binding
Protein Structure, Tertiary
Receptors, Immunologic / biosynthesis
Receptors, Immunologic / chemistry*
Receptors, Immunologic / genetics*
Receptors, Lipoprotein*
Receptors, Scavenger
Scavenger Receptors, Class A
Scavenger Receptors, Class B
Sequence Homology, Amino Acid
Staphylococcus aureus / metabolism
Tissue Distribution
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1(RCB0285) |
