RRC ID 72475
著者 Li Y, Zhang J, Liu YD, Zhou XY, Chen X, Zhe J, Zhang QY, Zhang XF, Chen YX, Wang Z, Chen SL.
タイトル Long non-coding RNA TUG1 and its molecular mechanisms in polycystic ovary syndrome.
ジャーナル RNA Biol
Abstract Polycystic ovary syndrome (PCOS) causes anovulatory infertility in women of reproductive age, but etiopathogenesis of PCOS remains undetermined. Taurine up-regulated 1 (TUG1), an evolutionarily conserved long non-coding RNA, performs various biological functions; however, the role of TUG1 in PCOS remains unclear. Herein, TUG1 expression was assayed in granulosa cells (GCs) of 100 patients with PCOS and 100 control participants. Receiver operating characteristic (ROC) curve analysis was conducted to determine the diagnostic value of TUG1 in PCOS. TUG1 expression was also silenced in KGN cells to explore the role of TUG1 in cellular proliferation, apoptosis, cell-cycle progression, autophagy, and steroidogenesis. We found that TUG1 levels were dramatically increased in the PCOS group compared with those of the control group; this increased expression was related to a rising antral follicle count (R = 0.209, P < 0.001 versus control). The ROC curve indicated a significant separation between PCOS group and the control group (AUC: 0.702; 95% CI: 0.630-0.773; P < 0.001). TUG1 showed a predominantly nuclear localization in human GCs. TUG1 knockdown reduced cellular proliferation, and promoted MAPKs pathway-dependent apoptosis and P21-dependent autophagy, but may not affect cell-cycle progression. TUG1 knockdown increased aromatase expression and oestradiol biosynthesis. Our results indicate that increased TUG1 expression in PCOS GCs may contribute to excessive follicular activation and growth, and may disrupt the selection of dominant follicle. Our study shows that TUG1 can be used as a diagnostic biomarker for PCOS.
巻・号 17(12)
ページ 1798-1810
公開日 2020-12-1
DOI 10.1080/15476286.2020.1783850
PMID 32559120
PMC PMC7714456
MeSH Adult Apoptosis / genetics Aromatase / metabolism Autophagy / genetics Biomarkers Case-Control Studies Cell Proliferation Cells, Cultured Disease Susceptibility Estradiol / biosynthesis Female Follicle Stimulating Hormone / blood Gene Expression Regulation* / drug effects Gene Knockdown Techniques Granulosa Cells / metabolism Humans Insulin / administration & dosage Insulin / therapeutic use MAP Kinase Signaling System Ovarian Follicle / metabolism Ovarian Follicle / pathology Polycystic Ovary Syndrome / diagnosis Polycystic Ovary Syndrome / drug therapy Polycystic Ovary Syndrome / genetics* Polycystic Ovary Syndrome / metabolism RNA, Long Noncoding / genetics* ROC Curve Young Adult
IF 5.35
リソース情報
ヒト・動物細胞 KGN(RCB1154)