RRC ID 728
Author Kitazawa D, Hatakeda Y, Kamada M, Fujii N, Miyazawa Y, Hoshino A, Iida S, Fukaki H, Morita MT, Tasaka M, Suge H, Takahashi H.
Title Shoot circumnutation and winding movements require gravisensing cells.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Circumnutation and winding in plants are universal growth movements that allow plants to survive despite their sessile nature. However, the detailed molecular mechanisms controlling these phenomena remain unclear. We previously found that a gravitropic mutant of Japanese morning glory (Pharbitis nil or Ipomoea nil), Shidare-asagao (weeping), is defective not only in circumnutation but also in the winding response. This phenotype is similar to that of the Arabidopsis SCARECROW (SCR) mutant. We therefore investigated whether morning glory SCR (PnSCR) is involved in the weeping phenotype. We found that one amino acid was inserted into the highly conserved VHIID motif in weeping-type PnSCR; this mutation caused abnormal endodermal differentiation. We introduced either the mutant or WT PnSCR into Arabidopsis scr mutants for complementation tests. PnSCR of the WT, but not of weeping, rescued the shoot gravitropism and circumnutation of scr. These results show that both the abnormal gravitropism and the circumnutation defect in weeping are attributable to a loss of PnSCR function. Thus, our data show that gravisensing endodermal cells are indispensable for shoot circumnutation and the winding response and that PnSCR is responsible for the abnormal phenotypes of weeping.
Volume 102(51)
Pages 18742-7
Published 2005-12-20
DOI 10.1073/pnas.0504617102
PII 0504617102
PMID 16339910
PMC PMC1310508
MeSH Amino Acid Sequence Arabidopsis / genetics Base Sequence Genetic Complementation Test Gravity Sensing / physiology* Ipomoea nil / cytology* Ipomoea nil / genetics Ipomoea nil / growth & development Ipomoea nil / physiology* Molecular Sequence Data Mutation / genetics Phenotype Plant Proteins / genetics Plant Proteins / metabolism Plant Shoots / cytology* Plant Shoots / genetics Plant Shoots / growth & development Plant Shoots / physiology* Plants, Genetically Modified
IF 9.504
Times Cited 38
Morning Glory