| RRC ID |
72809
|
| 著者 |
Oura S, Hino T, Satoh T, Noda T, Koyano T, Isotani A, Matsuyama M, Akira S, Ishiguro KI, Ikawa M.
|
| タイトル |
Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Meiosis is a hallmark event in germ cell development that accompanies sequential events executed by numerous molecules. Therefore, characterization of these factors is one of the best strategies to clarify the mechanism of meiosis. Here, we report tripartite motif-containing 41 (TRIM41), a ubiquitin ligase E3, as an essential factor for proper meiotic progression and fertility in male mice. Trim41 knockout (KO) spermatocytes exhibited synaptonemal complex protein 3 (SYCP3) overloading, especially on the X chromosome. Furthermore, mutant mice lacking the RING domain of TRIM41, required for the ubiquitin ligase E3 activity, phenocopied Trim41 KO mice. We then examined the behavior of mutant TRIM41 (ΔRING-TRIM41) and found that ΔRING-TRIM41 accumulated on the chromosome axes with overloaded SYCP3. This result suggested that TRIM41 exerts its function on the chromosome axes. Our study revealed that Trim41 is essential for preventing SYCP3 overloading, suggesting a TRIM41-mediated mechanism for regulating chromosome axis protein dynamics during male meiotic progression.
|
| 巻・号 |
18(6)
|
| ページ |
e1010241
|
| 公開日 |
2022-6-1
|
| DOI |
10.1371/journal.pgen.1010241
|
| PII |
PGENETICS-D-22-00112
|
| PMID |
35648791
|
| PMC |
PMC9191731
|
| MeSH |
Animals
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism
Male
Meiosis / genetics
Mice
Mice, Knockout
Nuclear Proteins* / genetics
Nuclear Proteins* / metabolism
Spermatocytes / metabolism
Synaptonemal Complex* / genetics
Synaptonemal Complex* / metabolism
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism*
|
| IF |
5.175
|
| リソース情報 |
| 遺伝子材料 |
pClgn1.1-Trim41-PA-1D4 (RDB19656) |
