Reference - Detail
| RRC ID | 72852 |
|---|---|
| Author | Hong Z, Kase Y, Moritake T, Gerelchuluun A, Sun L, Suzuki K, Terunuma T, Yasuoka K, Kumada H, Anzai K, Sakurai H, Sakae T, Tsuboi K. |
| Title | Lineal energy-based evaluation of oxidative DNA damage induced by proton beams and X-rays. |
| Journal | Int J Radiat Biol |
| Abstract |
PURPOSE:To determine the oxidative capabilities of proton beams compared to X-rays based on lineal energy (y). MATERIALS AND METHODS:Microdosimetry was used to determine y-values of 155 MeV protons. Salmon testes deoxyribonucleic acid (ST-DNA) in solution and human tumor cells (MOLT-4) were irradiated with 200 kV X-rays (X) or 155 MeV protons at their plateau (P) and near their Bragg-peak (B). 8-Hydroxydeoxyguanosine (8-OHdG) production was determined by high performance liquid chromatography. Double-strand breaks (DSB) in ST-DNA were evaluated by agarose gel electrophoresis and DSB in cell nuclei were evaluated by immunocytochemical analysis of phosphorylated histone H2AX (γH2AX) foci. Edaravone was used as a radical scavenger. RESULTS:8-OHdG yields in ST-DNA were significantly higher with X than with P or B, and they were significantly higher with P than with B. DSB yields in ST-DNA were higher with P than with B or X, although not statistically significant, and were nearly equal with B and X. Although γH2AX foci formation in MOLT-4 cells after each irradiation type was nearly identical, the addition of edaravone significantly inhibited foci formation only with X. CONCLUSIONS:Our results indicated that radical-induced indirect DNA damage was significantly lower with proton beams than with X-rays. |
| Volume | 89(1) |
| Pages | 36-43 |
| Published | 2013-1-1 |
| DOI | 10.3109/09553002.2012.715791 |
| PMID | 22901337 |
| MeSH | 8-Hydroxy-2'-Deoxyguanosine Animals Cell Line, Tumor DNA Breaks, Double-Stranded / radiation effects DNA Damage* Deoxyguanosine / analogs & derivatives Deoxyguanosine / metabolism Histones / metabolism Humans Linear Energy Transfer / radiation effects* Oxidative Stress / radiation effects* Photons / adverse effects Protons / adverse effects* Radiometry X-Rays / adverse effects |
| IF | 3.027 |
| Resource | |
| Human and Animal Cells | MOLT-4(RCB0206) |