論文 - 詳細
| RRC ID | 72899 |
|---|---|
| 著者 | Endoh H, Sasaki H, Maruyama K, Takeyama K, Waga I, Shimizu T, Kato S, Kawashima H. |
| タイトル | Rapid activation of MAP kinase by estrogen in the bone cell line. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We examined the effect of estrogen on mitogen-activated protein kinase (MAPK) in osteoblastic cells. Rat ROS 17/2.8 cells were exposed to 17beta-estradiol (E2) and MAPK activity in the cells was measured by an in vitro phosphorylation assay. E2 treatment caused a rapid and transient MAPK activation within 5 min. Insulin-like growth factor-I, which acts via their membrane receptors, caused a similar effect, but it required 10 min to reach the maximum level. Western blot analyses with anti-MAPK and anti-phosphotyrosine antibodies demonstrated that the E2 activation of MAPK was accompanied by phosphorylation of the enzyme. The concentration range (10 nM-1 pM) of E2 needed for this MAPK activation was less than that (1 microM-0.1 nM) needed for the transcriptional activation via the nuclear estrogen receptor (ER). These data provide the first evidence of MAPK activation by E2 through phosphorylation, which may be mediated through a putative plasma membrane receptor in the cultured bone cells. |
| 巻・号 | 235(1) |
| ページ | 99-102 |
| 公開日 | 1997-6-9 |
| DOI | 10.1006/bbrc.1997.6746 |
| PII | S0006291X97967462 |
| PMID | 9196043 |
| MeSH | Animals Antibodies / immunology Blotting, Western Calcium-Calmodulin-Dependent Protein Kinases / immunology Calcium-Calmodulin-Dependent Protein Kinases / metabolism* Cell Line Enzyme Activation Estradiol / pharmacology* Insulin-Like Growth Factor I / pharmacology Osteoblasts / drug effects Osteoblasts / enzymology* Phosphorylation Phosphotyrosine / immunology Rats Receptors, Estrogen / metabolism |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | ROS-17/2.8-5(RCB0462) |