RRC ID 72939
Author Hocquet C, Robellet X, Modolo L, Sun XM, Burny C, Cuylen-Haering S, Toselli E, Clauder-Münster S, Steinmetz L, Haering CH, Marguerat S, Bernard P.
Title Condensin controls cellular RNA levels through the accurate segregation of chromosomes instead of directly regulating transcription.
Journal Elife
Abstract Condensins are genome organisers that shape chromosomes and promote their accurate transmission. Several studies have also implicated condensins in gene expression, although any mechanisms have remained enigmatic. Here, we report on the role of condensin in gene expression in fission and budding yeasts. In contrast to previous studies, we provide compelling evidence that condensin plays no direct role in the maintenance of the transcriptome, neither during interphase nor during mitosis. We further show that the changes in gene expression in post-mitotic fission yeast cells that result from condensin inactivation are largely a consequence of chromosome missegregation during anaphase, which notably depletes the RNA-exosome from daughter cells. Crucially, preventing karyotype abnormalities in daughter cells restores a normal transcriptome despite condensin inactivation. Thus, chromosome instability, rather than a direct role of condensin in the transcription process, changes gene expression. This knowledge challenges the concept of gene regulation by canonical condensin complexes.
Volume 7
Published 2018-9-19
DOI 10.7554/eLife.38517
PII 38517
PMID 30230473
PMC PMC6173581
MeSH Adenosine Triphosphatases / genetics* Adenosine Triphosphatases / metabolism Chromosome Segregation / genetics* DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism G2 Phase / genetics Gene Expression Profiling Gene Expression Regulation, Fungal* Genomic Instability / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Confocal Multiprotein Complexes / genetics* Multiprotein Complexes / metabolism Mutation RNA, Fungal / genetics* RNA, Fungal / metabolism S Phase / genetics Schizosaccharomyces / genetics Schizosaccharomyces / metabolism
IF 7.08