RRC ID 72949
Author Seguchi K, Kataoka H, Uchino H, Nabeshima K, Koono M.
Title Secretion of protease nexin-II/amyloid beta protein precursor by human colorectal carcinoma cells and its modulation by cytokines/growth factors and proteinase inhibitors.
Journal Biol Chem
Abstract Trypsin inhibitors secreted by human colorectal adenocarcinoma cell lines were analyzed by reverse zymography. Among eleven cell lines analyzed, the major inhibitor secreted was protease nexin-II (PN-II), a secreted form of amyloid beta protein precursor (APP) containing a Kunitz-type serine proteinase inhibitor domain. Expression of the APP gene was also confirmed in the cell lines and the main APP mRNA species were PN-II types. The APP gene expression was constant during cell growth in vitro. On the other hand, the rate of extracellular PN-II accumulation markedly increased after long-term serum-free maintenance of the confluent culture. The extracellular accumulation of PN-II was also strongly stimulated either by interleukin-1beta (IL-1beta) treatment or to a lesser extent by basic fibroblast growth factor, tumor necrosis factor-alpha, hepatocyte growth factor or epidermal growth factor. Neither serum depletion- nor IL-1beta-induced stimulation of extracellular PN-II accumulation were accompanied by obvious alteration of the levels of APP mRNA and cellular APP holoprotein, suggesting that the enhanced extracellular accumulation of PN-II might result from up-regulation of the secretory pathway of APP. The IL-1beta-induced PN-II secretion was significantly inhibited by relatively high concentrations (50-200 microg/ml) of aprotinin, a serine proteinase inhibitor, in a dose-dependent manner without obvious cell-toxic effects.
Volume 380(4)
Pages 473-83
Published 1999-4-1
DOI 10.1515/BC.1999.061
PMID 10355633
MeSH Adenocarcinoma / enzymology Adenocarcinoma / metabolism Adenocarcinoma / pathology Amino Acid Sequence Amyloid beta-Protein Precursor / genetics Amyloid beta-Protein Precursor / metabolism* Base Sequence Carrier Proteins / metabolism* Colorectal Neoplasms / enzymology Colorectal Neoplasms / metabolism* Colorectal Neoplasms / pathology Culture Media, Serum-Free DNA Primers Growth Substances / pharmacology* Humans Interleukin-1 / pharmacology* Molecular Sequence Data Protease Nexins RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Cell Surface Trypsin Inhibitors / pharmacology* Tumor Cells, Cultured
IF 3.27
Human and Animal Cells LoVo(RCB1639)