Reference - Detail
| RRC ID | 72986 |
|---|---|
| Author | Cui Y, Cao W, He Y, Zhao Q, Wakazaki M, Zhuang X, Gao J, Zeng Y, Gao C, Ding Y, Wong HY, Wong WS, Lam HK, Wang P, Ueda T, Rojas-Pierce M, Toyooka K, Kang BH, Jiang L. |
| Title | A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells. |
| Journal | Nat Plants |
| Abstract |
Plant vacuoles are dynamic organelles that play essential roles in regulating growth and development. Two distinct models of vacuole biogenesis have been proposed: separate vacuoles are formed by the fusion of endosomes, or the single interconnected vacuole is derived from the endoplasmic reticulum. These two models are based on studies of two-dimensional (2D) transmission electron microscopy and 3D confocal imaging, respectively. Here, we performed 3D electron tomography at nanometre resolution to illustrate vacuole biogenesis in Arabidopsis root cells. The whole-cell electron tomography analysis first identified unique small vacuoles (SVs; 400-1,000 nm in diameter) as nascent vacuoles in early developmental cortical cells. These SVs contained intraluminal vesicles and were mainly derived/matured from multivesicular body (MVB) fusion. The whole-cell vacuole models and statistical analysis on wild-type root cells of different vacuole developmental stages demonstrated that central vacuoles were derived from MVB-to-SV transition and subsequent fusions of SVs. Further electron tomography analysis on mutants defective in MVB formation/maturation or vacuole fusion demonstrated that central vacuole formation required functional MVBs and membrane fusion machineries. |
| Volume | 5(1) |
| Pages | 95-105 |
| Published | 2019-1-1 |
| DOI | 10.1038/s41477-018-0328-1 |
| PII | 10.1038/s41477-018-0328-1 |
| PMID | 30559414 |
| MeSH | Arabidopsis / cytology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Cell Fusion Electron Microscope Tomography / methods* Guanine Nucleotide Exchange Factors / genetics Imaging, Three-Dimensional Multivesicular Bodies / metabolism Mutation Plant Cells Plant Roots / cytology* Plants, Genetically Modified SNARE Proteins / metabolism Vacuoles / physiology* Vesicular Transport Proteins / genetics |
| IF | 13.256 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pst05666 pst18264 |