RRC ID 73060
Author Zheng F, Dong F, Yu S, Li T, Jian Y, Nie L, Fu C.
Title Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.
Journal J Biol Chem
Abstract The spindle apparatus segregates bi-oriented sister chromatids during mitosis but mono-oriented homologous chromosomes during meiosis I. It has remained unclear if similar molecular mechanisms operate to regulate spindle dynamics during mitosis and meiosis I. Here, we employed live-cell microscopy to compare the spindle dynamics of mitosis and meiosis I in fission yeast cells and demonstrated that the conserved kinesin-14 motor Klp2 plays a specific role in maintaining metaphase spindle length during meiosis I but not during mitosis. Moreover, the maintenance of metaphase spindle stability during meiosis I requires the synergism between Klp2 and the conserved microtubule cross-linker Ase1, as the absence of both proteins causes exacerbated defects in metaphase spindle stability. The synergism is not necessary for regulating mitotic spindle dynamics. Hence, our work reveals a new molecular mechanism underlying meiotic spindle dynamics and provides insights into understanding differential regulation of meiotic and mitotic events.
Volume 295(38)
Pages 13287-13298
Published 2020-9-18
DOI 10.1074/jbc.RA120.012905
PII S0021-9258(17)50044-7
PMID 32723864
PMC PMC7504916
MeSH Metaphase* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / genetics Spindle Apparatus / metabolism*
IF 4.238