論文 - 詳細
| RRC ID | 73060 |
|---|---|
| 著者 | Zheng F, Dong F, Yu S, Li T, Jian Y, Nie L, Fu C. |
| タイトル | Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I. |
| ジャーナル | J Biol Chem |
| Abstract |
The spindle apparatus segregates bi-oriented sister chromatids during mitosis but mono-oriented homologous chromosomes during meiosis I. It has remained unclear if similar molecular mechanisms operate to regulate spindle dynamics during mitosis and meiosis I. Here, we employed live-cell microscopy to compare the spindle dynamics of mitosis and meiosis I in fission yeast cells and demonstrated that the conserved kinesin-14 motor Klp2 plays a specific role in maintaining metaphase spindle length during meiosis I but not during mitosis. Moreover, the maintenance of metaphase spindle stability during meiosis I requires the synergism between Klp2 and the conserved microtubule cross-linker Ase1, as the absence of both proteins causes exacerbated defects in metaphase spindle stability. The synergism is not necessary for regulating mitotic spindle dynamics. Hence, our work reveals a new molecular mechanism underlying meiotic spindle dynamics and provides insights into understanding differential regulation of meiotic and mitotic events. |
| 巻・号 | 295(38) |
| ページ | 13287-13298 |
| 公開日 | 2020-9-18 |
| DOI | 10.1074/jbc.RA120.012905 |
| PII | S0021-9258(17)50044-7 |
| PMID | 32723864 |
| PMC | PMC7504916 |
| MeSH | Metaphase* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / genetics Spindle Apparatus / metabolism* |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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