RRC ID |
73060
|
著者 |
Zheng F, Dong F, Yu S, Li T, Jian Y, Nie L, Fu C.
|
タイトル |
Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.
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ジャーナル |
J Biol Chem
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Abstract |
The spindle apparatus segregates bi-oriented sister chromatids during mitosis but mono-oriented homologous chromosomes during meiosis I. It has remained unclear if similar molecular mechanisms operate to regulate spindle dynamics during mitosis and meiosis I. Here, we employed live-cell microscopy to compare the spindle dynamics of mitosis and meiosis I in fission yeast cells and demonstrated that the conserved kinesin-14 motor Klp2 plays a specific role in maintaining metaphase spindle length during meiosis I but not during mitosis. Moreover, the maintenance of metaphase spindle stability during meiosis I requires the synergism between Klp2 and the conserved microtubule cross-linker Ase1, as the absence of both proteins causes exacerbated defects in metaphase spindle stability. The synergism is not necessary for regulating mitotic spindle dynamics. Hence, our work reveals a new molecular mechanism underlying meiotic spindle dynamics and provides insights into understanding differential regulation of meiotic and mitotic events.
|
巻・号 |
295(38)
|
ページ |
13287-13298
|
公開日 |
2020-9-18
|
DOI |
10.1074/jbc.RA120.012905
|
PII |
S0021-9258(17)50044-7
|
PMID |
32723864
|
PMC |
PMC7504916
|
MeSH |
Metaphase*
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / metabolism*
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Spindle Apparatus / genetics
Spindle Apparatus / metabolism*
|
IF |
4.238
|
リソース情報 |
酵母 |
|