RRC ID 73232
Author Taguchi T, Kato Y, Baba Y, Nishimura G, Tanigaki Y, Horiuchi C, Mochimatsu I, Tsukuda M.
Title Protein levels of p21, p27, cyclin E and Bax predict sensitivity to cisplatin and paclitaxel in head and neck squamous cell carcinomas.
Journal Oncol Rep
Abstract Regression of tumor mass by chemotherapy is caused by growth suppression and/or apoptosis of tumor cells. Therefore, expression levels of cell cycle molecules and apoptosis should be predictive markers for the efficacy of a drug. In the present study, the relationship between expression of molecules in the cell cycle and apoptosis and chemosensitivity was investigated in head and neck squamous cell carcinoma cell lines. Expression of p53, p21, p27, cyclin D1, cyclin E, and Bax in 17 such cell lines were analyzed by Western blot analysis. The concentrations of four chemotherapeutic agents (cisplatin, 5-FU, vincristine, and paclitaxel) resulting in 50% cell growth inhibition were calculated as IC50 values for each cell line. Cell cycle analysis was performed using a FACScan flow cytometer. Cells with strong expression of p21, p27, or Bax showed significantly higher sensitivity to cisplatin, and cells with strong expression of Bax or weak expression of cyclin E showed significantly higher sensitivity to paclitaxel. Cisplatin most effectively killed cells expressing both p21 and p27 or either at G1 phase. Though the assessments of p21, p27, Bax, and cyclin E expression in tumor tissues have been reported to be useful as prognostic factors in head and neck squamous cell carcinoma, these correlations might not only describe the malignant biological behavior of the tumor, but also the response to chemotherapy. Furthermore, p21/p27 expression might be a useful guide for the choice of chemotherapeutic agents.
Volume 11(2)
Pages 421-6
Published 2004-2-1
PMID 14719078
MeSH Aged Carcinoma, Squamous Cell / pathology Cell Survival / drug effects Cisplatin / toxicity* Cyclin E / metabolism* Cyclin-Dependent Kinase Inhibitor p21 Cyclins / metabolism* DNA Primers Estrogen Receptor alpha Fluorouracil / toxicity G1 Phase / drug effects Head and Neck Neoplasms / pathology Humans Middle Aged Neoplasm Staging Paclitaxel / toxicity* Proliferating Cell Nuclear Antigen / metabolism* Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-bcl-2* Receptors, Estrogen / metabolism Receptors, Progesterone / metabolism Tumor Suppressor Protein p53 / genetics Vincristine / toxicity bcl-2-Associated X Protein
IF 3.417
Human and Animal Cells WI-38