論文 - 詳細
| RRC ID | 73292 |
|---|---|
| 著者 | Krueger D, Quinkler T, Mortensen SA, Sachse C, De Renzis S. |
| タイトル | Cross-linker-mediated regulation of actin network organization controls tissue morphogenesis. |
| ジャーナル | J Cell Biol |
| Abstract |
Contraction of cortical actomyosin networks driven by myosin activation controls cell shape changes and tissue morphogenesis during animal development. In vitro studies suggest that contractility also depends on the geometrical organization of actin filaments. Here we analyze the function of actomyosin network topology in vivo using optogenetic stimulation of myosin-II in Drosophila embryos. We show that early during cellularization, hexagonally arrayed actomyosin fibers are resilient to myosin-II activation. Actomyosin fibers then acquire a ring-like conformation and become contractile and sensitive to myosin-II. This transition is controlled by Bottleneck, a Drosophila unique protein expressed for only a short time during early cellularization, which we show regulates actin bundling. In addition, it requires two opposing actin cross-linkers, Filamin and Fimbrin. Filamin acts synergistically with Bottleneck to facilitate hexagonal patterning, while Fimbrin controls remodeling of the hexagonal network into contractile rings. Thus, actin cross-linking regulates the spatio-temporal organization of actomyosin contraction in vivo, which is critical for tissue morphogenesis. |
| 巻・号 | 218(8) |
| ページ | 2743-2761 |
| 公開日 | 2019-8-5 |
| DOI | 10.1083/jcb.201811127 |
| PII | jcb.201811127 |
| PMID | 31253650 |
| PMC | PMC6683744 |
| MeSH | Actins / metabolism* Actomyosin / metabolism Animals Cross-Linking Reagents / metabolism* Drosophila Proteins / chemistry Drosophila Proteins / metabolism Drosophila melanogaster / embryology* Drosophila melanogaster / metabolism* Elasticity Embryo, Nonmammalian / metabolism Gastrulation HeLa Cells Humans Models, Biological Morphogenesis* Mutation / genetics Myosin Type II / metabolism Optogenetics Phenotype Protein Domains |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 52 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#115092 DGRC#115514 |