論文 - 詳細
| RRC ID | 73317 |
|---|---|
| 著者 | Wei W, Zheng B, Zheng S, Wu D, Chu Y, Zhang S, Wang D, Ma X, Liu X, Yao X, Fu C. |
| タイトル | The Cdc42 GAP Rga6 promotes monopolar outgrowth of spores. |
| ジャーナル | J Cell Biol |
| Abstract |
The molecular mechanisms underlying the establishment of the monopolar growth of fission yeast spores have been less characterized. Here, we report that the Cdc42 GTPase-activating protein (GAP) Rga6 is required for promoting monopolar growth during spore germination. The absence of Rga6 increases the number of spores that grow in a bipolar fashion. Rga6 decorates the non-growing cortical region, binds phosphatidylinositol 4,5-bisphosphate, and colocalizes with the phosphatidylinositol 4,5-bisphosphate-binding protein Opy1. Overexpression of Opy1 diminishes the cortical localization of Rga6. The characteristic localization of Rga6 on the cell cortex depends on the C-terminal PBR region of Rga6. Moreover, engineered chimera composed of the Rga6 C-terminal PBR region fused to the GAP domain of Rga3 or Rga4 are sufficient to rescue the spore growth phenotype caused by the absence of Rga6. Hence, our work establishes a paradigm in which the lipid composition of the plasma membrane directs polarized cell growth by specifying the cortical localization of a GAP protein. |
| 巻・号 | 222(1) |
| 公開日 | 2023-1-2 |
| DOI | 10.1083/jcb.202202064 |
| PII | 213678 |
| PMID | 36355349 |
| PMC | PMC9652770 |
| MeSH | GTPase-Activating Proteins / metabolism Phosphatidylinositol 4,5-Diphosphate / metabolism Schizosaccharomyces* / growth & development Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism Spores, Fungal* / genetics Spores, Fungal* / growth & development cdc42 GTP-Binding Protein / genetics cdc42 GTP-Binding Protein / metabolism |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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