| RRC ID |
73317
|
| 著者 |
Wei W, Zheng B, Zheng S, Wu D, Chu Y, Zhang S, Wang D, Ma X, Liu X, Yao X, Fu C.
|
| タイトル |
The Cdc42 GAP Rga6 promotes monopolar outgrowth of spores.
|
| ジャーナル |
J Cell Biol
|
| Abstract |
The molecular mechanisms underlying the establishment of the monopolar growth of fission yeast spores have been less characterized. Here, we report that the Cdc42 GTPase-activating protein (GAP) Rga6 is required for promoting monopolar growth during spore germination. The absence of Rga6 increases the number of spores that grow in a bipolar fashion. Rga6 decorates the non-growing cortical region, binds phosphatidylinositol 4,5-bisphosphate, and colocalizes with the phosphatidylinositol 4,5-bisphosphate-binding protein Opy1. Overexpression of Opy1 diminishes the cortical localization of Rga6. The characteristic localization of Rga6 on the cell cortex depends on the C-terminal PBR region of Rga6. Moreover, engineered chimera composed of the Rga6 C-terminal PBR region fused to the GAP domain of Rga3 or Rga4 are sufficient to rescue the spore growth phenotype caused by the absence of Rga6. Hence, our work establishes a paradigm in which the lipid composition of the plasma membrane directs polarized cell growth by specifying the cortical localization of a GAP protein.
|
| 巻・号 |
222(1)
|
| 公開日 |
2023-1-2
|
| DOI |
10.1083/jcb.202202064
|
| PII |
213678
|
| PMID |
36355349
|
| PMC |
PMC9652770
|
| MeSH |
GTPase-Activating Proteins / metabolism
Phosphatidylinositol 4,5-Diphosphate / metabolism
Schizosaccharomyces* / growth & development
Schizosaccharomyces* / metabolism
Schizosaccharomyces pombe Proteins* / genetics
Schizosaccharomyces pombe Proteins* / metabolism
Spores, Fungal* / genetics
Spores, Fungal* / growth & development
cdc42 GTP-Binding Protein / genetics
cdc42 GTP-Binding Protein / metabolism
|
| IF |
8.811
|
| リソース情報 |
| 酵母 |
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