RRC ID 73396
著者 Noda T.
タイトル The maternal genes Ci-p53/p73-a and Ci-p53/p73-b regulate zygotic ZicL expression and notochord differentiation in Ciona intestinalis embryos.
ジャーナル Dev Biol
Abstract I isolated a Ciona intestinalis homolog of p53, Ci-p53/p73-a, in a microarray screen of rapidly degraded maternal mRNA by comparing the transcriptomes of unfertilized eggs and 32-cell stage embryos. Higher expression of the gene in eggs and lower expression in later embryonic stages were confirmed by whole-mount in situ hybridization (WISH) and quantitative reverse transcription-PCR (qRT-PCR); expression was ubiquitous in eggs and early embryos. Knockdown of Ci-p53/p73-a by injection of antisense morpholino oligonucleotides (MOs) severely perturbed gastrulation cell movements and expression of notochord marker genes. A key regulator of notochord differentiation in Ciona embryos is Brachyury (Ci-Bra), which is directly activated by a zic-like gene (Ci-ZicL). The expression of Ci-ZicL and Ci-Bra in A-line notochord precursors was downregulated in Ci-p53/p73-a knockdown embryos. Maternal expression of Ci-p53/p73-b, a homolog of Ci-p53/p73-a, was also detected. In Ci-p53/p73-b knockdown embryos, gastrulation cell movements, expression of Ci-ZicL and Ci-Bra in A-line notochord precursors, and expression of notochord marker gene at later stages were perturbed. The upstream region of Ci-ZicL contains putative p53-binding sites. Cis-regulatory analysis of Ci-ZicL showed that these sites are involved in expression of Ci-ZicL in A-line notochord precursors at the 32-cell and early gastrula stages. These results suggest that p53 genes are maternal factors that play a crucial role in A-line notochord differentiation in C. intestinalis embryos by regulating Ci-ZicL expression.
巻・号 360(1)
ページ 216-29
公開日 2011-12-1
DOI 10.1016/j.ydbio.2011.08.029
PII S0012-1606(11)01229-2
PMID 21925489
MeSH Animals Base Sequence Cell Differentiation Ciona intestinalis / cytology Ciona intestinalis / embryology* Ciona intestinalis / genetics* Ciona intestinalis / metabolism DNA Primers / genetics DNA-Binding Proteins / genetics* Evolution, Molecular Female Fetal Proteins / genetics Gastrulation / genetics Gene Expression Regulation, Developmental Gene Knockdown Techniques Gene Regulatory Networks Genes, p53* Male Molecular Sequence Data Neurogenesis / genetics Notochord / cytology Notochord / embryology* Notochord / metabolism Nuclear Proteins / genetics* RNA, Messenger / genetics RNA, Messenger / metabolism T-Box Domain Proteins / genetics Tumor Protein p73 Tumor Suppressor Proteins / genetics* Zygote / metabolism
IF 2.896
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int