RRC ID 73400
著者 Hamada M, Shimozono N, Ohta N, Satou Y, Horie T, Kawada T, Satake H, Sasakura Y, Satoh N.
タイトル Expression of neuropeptide- and hormone-encoding genes in the Ciona intestinalis larval brain.
ジャーナル Dev Biol
Abstract Despite containing only approximately 330 cells, the central nervous system (CNS) of Ciona intestinalis larvae has an architecture that is similar to the vertebrate CNS. Although only vertebrates have a distinct hypothalamus-the source of numerous neurohormone peptides that play pivotal roles in the development, function, and maintenance of various neuronal and endocrine systems, it is suggested that the Ciona brain contains a region that corresponds to the vertebrate hypothalamus. To identify genes expressed in the brain, we isolated brain vesicles using transgenic embryos carrying Ci-β-tubulin(promoter)::Kaede, which resulted in robust Kaede expression in the larval CNS. The associated transcriptome was investigated using microarray analysis. We identified 565 genes that were preferentially expressed in the larval brain. Among these genes, 11 encoded neurohormone peptides including such hypothalamic peptides as gonadotropin-releasing hormone and oxytocin/vasopressin. Six of the identified peptide genes had not been previously described. We also found that genes encoding receptors for some of the peptides were expressed in the brain. Interestingly, whole-mount in situ hybridization showed that most of the peptide genes were expressed in the ventral brain. This catalog of the genes expressed in the larval brain should help elucidate the evolution, development, and functioning of the chordate brain.
巻・号 352(2)
ページ 202-14
公開日 2011-4-15
DOI 10.1016/j.ydbio.2011.01.006
PII S0012-1606(11)00022-4
PMID 21237141
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Brain / growth & development* Brain / metabolism* Ciona intestinalis / genetics* Ciona intestinalis / growth & development* Ciona intestinalis / metabolism Evolution, Molecular Gene Expression Regulation, Developmental Hypothalamus / growth & development Hypothalamus / metabolism In Situ Hybridization Invertebrate Hormones / genetics Larva / growth & development Larva / metabolism Molecular Sequence Data Neuropeptides / genetics Oligonucleotide Array Sequence Analysis Receptors, G-Protein-Coupled / genetics Receptors, Neuropeptide / genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / genetics Transcription Factors / genetics Vertebrates / growth & development Vertebrates / metabolism
IF 2.896
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int