Reference - Detail
| RRC ID | 73747 |
|---|---|
| Author | Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T. |
| Title | Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. |
| Journal | Nat Methods |
| Abstract |
We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks. |
| Volume | 7(9) |
| Pages | 729-32 |
| Published | 2010-9-1 |
| DOI | 10.1038/nmeth.1488 |
| PII | nmeth.1488 |
| PMID | 20693999 |
| MeSH | Animals Calcium / analysis* Calcium / metabolism Dictyostelium Fluorescent Dyes / analysis Fluorescent Dyes / chemistry Indicators and Reagents / analysis Indicators and Reagents / chemistry Mice Molecular Sequence Data Neurons / metabolism Signal Transduction Zebrafish |
| IF | 30.822 |
| Resource | |
| DNA material | Yellow Cameleon-Nano140/pcDNA3 (RDB19876) Yellow Cameleon-Nano65/pcDNA3 (RDB19877) Yellow Cameleon-Nano50/pcDNA3 (RDB19878) Yellow Cameleon-Nano30/pcDNA3 (RDB19879) Yellow Cameleon-Nano15/pcDNA3 (RDB19880) |