| RRC ID |
73753
|
| 著者 |
Gunawardane LS, Saito K, Nishida KM, Miyoshi K, Kawamura Y, Nagami T, Siomi H, Siomi MC.
|
| タイトル |
A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila.
|
| ジャーナル |
Science
|
| Abstract |
In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AGO3. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5' ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.
|
| 巻・号 |
315(5818)
|
| ページ |
1587-90
|
| 公開日 |
2007-3-16
|
| DOI |
10.1126/science.1140494
|
| PII |
1140494
|
| PMID |
17322028
|
| MeSH |
Amino Acid Sequence
Animals
Argonaute Proteins
Drosophila Proteins / chemistry
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / embryology
Drosophila melanogaster / genetics*
Drosophila melanogaster / metabolism
Female
Gene Library
Male
Models, Genetic
Molecular Sequence Data
Ovary / metabolism
Peptide Initiation Factors / chemistry
Peptide Initiation Factors / genetics
Peptide Initiation Factors / metabolism*
Proteins / genetics
Proteins / metabolism
RNA Interference
RNA, Small Interfering / chemistry
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism*
RNA-Induced Silencing Complex
Recombinant Fusion Proteins / metabolism
Retroelements
Testis / metabolism
|
| IF |
41.846
|
| リソース情報 |
| ショウジョウバエ |
10002Ab-1 |
