論文 - 詳細
| RRC ID | 73758 |
|---|---|
| 著者 | Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K. |
| タイトル | Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons. |
| ジャーナル | Mol Cell |
| Abstract |
PIWI-interacting RNAs (piRNAs) mediate transcriptional and post-transcriptional silencing of transposable element (TE) in animal gonads. In Drosophila ovaries, Piwi-piRNA complexes (Piwi-piRISCs) repress TE transcription by modifying the chromatin state, such as by H3K9 trimethylation. Here, we demonstrate that Piwi physically interacts with linker histone H1. Depletion of Piwi decreases H1 density at a subset of TEs, leading to their derepression. Silencing at these loci separately requires H1 and H3K9me3 and heterochromatin protein 1a (HP1a). Loss of H1 increases target loci chromatin accessibility without affecting H3K9me3 density at these loci, while loss of HP1a does not impact H1 density. Thus, Piwi-piRISCs require both H1 and HP1a to repress TEs, and the silencing is correlated with the chromatin state rather than H3K9me3 marks. These findings suggest that Piwi-piRISCs regulate the interaction of chromatin components with target loci to maintain silencing of TEs through the modulation of chromatin accessibility. |
| 巻・号 | 63(3) |
| ページ | 408-19 |
| 公開日 | 2016-8-4 |
| DOI | 10.1016/j.molcel.2016.06.008 |
| PII | S1097-2765(16)30239-8 |
| PMID | 27425411 |
| MeSH | Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism* Cells, Cultured Chromatin Assembly and Disassembly* Chromobox Protein Homolog 5 Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism DNA Methylation DNA Transposable Elements* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Epigenetic Repression* Female Histones / genetics Histones / metabolism* Ovary / cytology Ovary / metabolism* RNA Interference RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Transfection |
| IF | 15.584 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 10008Ab-1 |