| RRC ID |
73758
|
| 著者 |
Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K.
|
| タイトル |
Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons.
|
| ジャーナル |
Mol Cell
|
| Abstract |
PIWI-interacting RNAs (piRNAs) mediate transcriptional and post-transcriptional silencing of transposable element (TE) in animal gonads. In Drosophila ovaries, Piwi-piRNA complexes (Piwi-piRISCs) repress TE transcription by modifying the chromatin state, such as by H3K9 trimethylation. Here, we demonstrate that Piwi physically interacts with linker histone H1. Depletion of Piwi decreases H1 density at a subset of TEs, leading to their derepression. Silencing at these loci separately requires H1 and H3K9me3 and heterochromatin protein 1a (HP1a). Loss of H1 increases target loci chromatin accessibility without affecting H3K9me3 density at these loci, while loss of HP1a does not impact H1 density. Thus, Piwi-piRISCs require both H1 and HP1a to repress TEs, and the silencing is correlated with the chromatin state rather than H3K9me3 marks. These findings suggest that Piwi-piRISCs regulate the interaction of chromatin components with target loci to maintain silencing of TEs through the modulation of chromatin accessibility.
|
| 巻・号 |
63(3)
|
| ページ |
408-19
|
| 公開日 |
2016-8-4
|
| DOI |
10.1016/j.molcel.2016.06.008
|
| PII |
S1097-2765(16)30239-8
|
| PMID |
27425411
|
| MeSH |
Animals
Argonaute Proteins / genetics
Argonaute Proteins / metabolism*
Cells, Cultured
Chromatin Assembly and Disassembly*
Chromobox Protein Homolog 5
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism
DNA Methylation
DNA Transposable Elements*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / cytology
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism*
Epigenetic Repression*
Female
Histones / genetics
Histones / metabolism*
Ovary / cytology
Ovary / metabolism*
RNA Interference
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Transfection
|
| IF |
15.584
|
| リソース情報 |
| ショウジョウバエ |
10008Ab-1 |
