| RRC ID |
73760
|
| Author |
Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T, Nagai T.
|
| Title |
Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging.
|
| Journal |
ACS Chem Biol
|
| Abstract |
Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser.
|
| Volume |
13(8)
|
| Pages |
1938-1943
|
| Published |
2018-8-17
|
| DOI |
10.1021/acschembio.8b00200
|
| PMID |
29963852
|
| MeSH |
Escherichia coli
Fluorescence
Fluorescent Dyes / chemistry*
Fluorescent Dyes / radiation effects
HeLa Cells
Heating
Humans
Lasers
Light
Luminescent Proteins / chemistry*
Luminescent Proteins / genetics
Luminescent Proteins / radiation effects
Microscopy, Fluorescence / instrumentation
Microscopy, Fluorescence / methods
Mutagenesis
|
| IF |
4.434
|
| Resource |
| DNA material |
SPOON/pcDNA3 (RDB19863) |
