Reference - Detail
| RRC ID | 73760 |
|---|---|
| Author | Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T, Nagai T. |
| Title | Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. |
| Journal | ACS Chem Biol |
| Abstract |
Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser. |
| Volume | 13(8) |
| Pages | 1938-1943 |
| Published | 2018-8-17 |
| DOI | 10.1021/acschembio.8b00200 |
| PMID | 29963852 |
| MeSH | Escherichia coli Fluorescence Fluorescent Dyes / chemistry* Fluorescent Dyes / radiation effects HeLa Cells Heating Humans Lasers Light Luminescent Proteins / chemistry* Luminescent Proteins / genetics Luminescent Proteins / radiation effects Microscopy, Fluorescence / instrumentation Microscopy, Fluorescence / methods Mutagenesis |
| IF | 4.434 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 38 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | SPOON/pcDNA3 (RDB19863) |