論文 - 詳細
| RRC ID | 74156 |
|---|---|
| 著者 | Shoji K, Umemura Y, Katsuma S, Tomari Y. |
| タイトル | The piRNA cluster torimochi is an expanding transposon in cultured silkworm cells. |
| ジャーナル | PLoS Genet |
| Abstract |
PIWI proteins and PIWI-interacting RNAs (piRNAs) play a central role in repressing transposable elements in animal germ cells. It is thought that piRNAs are mainly produced from discrete genomic loci named piRNA clusters, which often contain many "dead" transposon remnants from past invasions and have heterochromatic features. In the genome of silkworm ovary-derived cultured cells called BmN4, a well-established model for piRNA research, torimochi was previously annotated as a unique and specialized genomic region that can capture transgenes and produce new piRNAs bearing a trans-silencing activity. However, the sequence identity of torimochi has remained elusive. Here, we carefully characterized torimochi by utilizing the updated silkworm genome sequence and the long-read sequencer MinION. We found that torimochi is in fact a full-length gypsy-like LTR retrotransposon, which is exceptionally active and has massively expanded its copy number in BmN4 cells. Many copies of torimochi in BmN4 cells have features of open chromatin and the ability to produce piRNAs. Therefore, torimochi may represent a young, growing piRNA cluster, which is still "alive" and active in transposition yet capable of trapping other transposable elements to produce de novo piRNAs. |
| 巻・号 | 19(2) |
| ページ | e1010632 |
| 公開日 | 2023-2-9 |
| DOI | 10.1371/journal.pgen.1010632 |
| PII | PGENETICS-D-22-01104 |
| PMID | 36758066 |
| PMC | PMC9946225 |
| MeSH | Animals Argonaute Proteins / genetics Bombyx* / genetics Cells, Cultured DNA Transposable Elements Female Germ Cells / metabolism Piwi-Interacting RNA* RNA, Small Interfering / genetics |
| IF | 5.175 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p50 |