RRC ID 74500
Author Taniguchi K, Igaki T.
Title Sas-Ptp10D shapes germ-line stem cell niche by facilitating JNK-mediated apoptosis.
Journal PLoS Genet
Abstract The function of the stem cell system is supported by a stereotypical shape of the niche structure. In Drosophila ovarian germarium, somatic cap cells form a dish-like niche structure that allows only two or three germ-line stem cells (GSCs) reside in the niche. Despite extensive studies on the mechanism of stem cell maintenance, the mechanisms of how the dish-like niche structure is shaped and how this structure contributes to the stem cell system have been elusive. Here, we show that a transmembrane protein Stranded at second (Sas) and its receptor Protein tyrosine phosphatase 10D (Ptp10D), effectors of axon guidance and cell competition via epidermal growth factor receptor (Egfr) inhibition, shape the dish-like niche structure by facilitating c-Jun N-terminal kinase (JNK)-mediated apoptosis. Loss of Sas or Ptp10D in gonadal apical cells, but not in GSCs or cap cells, during the pre-pupal stage results in abnormal shaping of the niche structure in the adult, which allows excessive, four to six GSCs reside in the niche. Mechanistically, loss of Sas-Ptp10D elevates Egfr signaling in the gonadal apical cells, thereby suppressing their naturally-occurring JNK-mediated apoptosis that is essential for the shaping of the dish-like niche structure by neighboring cap cells. Notably, the abnormal niche shape and resulting excessive GSCs lead to diminished egg production. Our data propose a concept that the stereotypical shaping of the niche structure optimizes the stem cell system, thereby maximizing the reproductive capacity.
Volume 19(3)
Pages e1010684
Published 2023-3-1
DOI 10.1371/journal.pgen.1010684
PMID 36972315
PMC PMC10079222
MeSH Animals Apoptosis / genetics Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism ErbB Receptors / genetics ErbB Receptors / metabolism Germ Cells / metabolism Phosphoric Monoester Hydrolases / metabolism Proto-Oncogene Proteins c-jun / metabolism Stem Cell Niche / genetics
IF 5.175
Drosophila DGRC#104055