RRC ID 74596
Author Yamaguchi S, Ikeda R, Umeda Y, Kosaka T, Yamahira S, Okamoto A.
Title Chemoenzymatic Labeling to Visualize Intercellular Contacts Using Lipidated SortaseA.
Journal Chembiochem
Abstract Methods to label intercellular contact have attracted attention because of their potential in cell biological and medical applications for the analysis of intercellular communications. In this study, a simple and versatile method for chemoenzymatic labeling of intercellularly contacting cells is demonstrated using a cell-surface anchoring reagent of a poly(ethylene glycol)(PEG)-lipid conjugate. The surface of each cell in the cell pairs of interest were decorated with sortase A (SrtA) and triglycine peptide that were lipidated with PEG-lipid. In the mixture of the two-cell populations, the triglycine-modified cells were enzymatically labeled with a fluorescent labeling reagent when in contact with SrtA-modified cells on a substrate. The selective labeling of the contacting cells was confirmed by confocal microscopy. The method is a promising tool for selective visualization of intercellularly contacting cells in cell mixtures for cell-cell communication analysis.
Volume 23(21)
Pages e202200474
Published 2022-11-4
DOI 10.1002/cbic.202200474
PMID 35976800
MeSH Aminoacyltransferases* / metabolism Bacterial Proteins / metabolism Cell Membrane / metabolism Lipids Microscopy, Confocal Peptides / metabolism Polyethylene Glycols / metabolism
IF 2.576
Resource
Human and Animal Cells Jurkat(RCB3052)