論文 - 詳細
| RRC ID | 74596 |
|---|---|
| 著者 | Yamaguchi S, Ikeda R, Umeda Y, Kosaka T, Yamahira S, Okamoto A. |
| タイトル | Chemoenzymatic Labeling to Visualize Intercellular Contacts Using Lipidated SortaseA. |
| ジャーナル | Chembiochem |
| Abstract |
Methods to label intercellular contact have attracted attention because of their potential in cell biological and medical applications for the analysis of intercellular communications. In this study, a simple and versatile method for chemoenzymatic labeling of intercellularly contacting cells is demonstrated using a cell-surface anchoring reagent of a poly(ethylene glycol)(PEG)-lipid conjugate. The surface of each cell in the cell pairs of interest were decorated with sortase A (SrtA) and triglycine peptide that were lipidated with PEG-lipid. In the mixture of the two-cell populations, the triglycine-modified cells were enzymatically labeled with a fluorescent labeling reagent when in contact with SrtA-modified cells on a substrate. The selective labeling of the contacting cells was confirmed by confocal microscopy. The method is a promising tool for selective visualization of intercellularly contacting cells in cell mixtures for cell-cell communication analysis. |
| 巻・号 | 23(21) |
| ページ | e202200474 |
| 公開日 | 2022-11-4 |
| DOI | 10.1002/cbic.202200474 |
| PMID | 35976800 |
| MeSH | Aminoacyltransferases* / metabolism Bacterial Proteins / metabolism Cell Membrane / metabolism Lipids Microscopy, Confocal Peptides / metabolism Polyethylene Glycols / metabolism |
| IF | 2.576 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB3052) |