論文 - 詳細
| RRC ID | 74672 |
|---|---|
| 著者 | Bu S, Tang Q, Wang Y, Lau SSY, Yong WL, Yu F. |
| タイトル | Drosophila CLASP regulates microtubule orientation and dendrite pruning by suppressing Par-1 kinase. |
| ジャーナル | Cell Rep |
| Abstract |
The evolutionarily conserved CLASPs (cytoplasmic linker-associated proteins) are microtubule-associated proteins that inhibit microtubule catastrophe and promote rescue. CLASPs can regulate axonal elongation and dendrite branching in growing neurons. However, their roles in microtubule orientation and neurite pruning in remodeling neurons remain unknown. Here, we identify the Drosophila CLASP homolog Orbit/MAST, which is required for dendrite pruning in ddaC sensory neurons during metamorphosis. Orbit is important for maintenance of the minus-end-out microtubule orientation in ddaC dendrites. Our structural analysis reveals that the microtubule lattice-binding TOG2 domain is required for Orbit to regulate dendritic microtubule orientation and dendrite pruning. In a genetic modifier screen, we further identify the conserved Par-1 kinase as a suppressor of Orbit in dendritic microtubule orientation. Moreover, elevated Par-1 function impairs dendritic microtubule orientation and dendrite pruning, phenocopying orbit mutants. Overall, our study demonstrates that Drosophila CLASP governs dendritic microtubule orientation and dendrite pruning at least partly via suppressing Par-1 kinase. |
| 巻・号 | 39(9) |
| ページ | 110887 |
| 公開日 | 2022-5-31 |
| DOI | 10.1016/j.celrep.2022.110887 |
| PII | S2211-1247(22)00662-3 |
| PMID | 35649352 |
| MeSH | Animals Drosophila* / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Glycogen Synthase Kinase 3 / metabolism Metamorphosis, Biological Microtubules / metabolism Neuronal Plasticity / genetics |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | GL00367 |