論文 - 詳細
| RRC ID | 75047 |
|---|---|
| 著者 | Yuki R, Ikeda Y, Yasutake R, Saito Y, Nakayama Y. |
| タイトル | SH2D4A promotes centrosome maturation to support spindle microtubule formation and mitotic progression. |
| ジャーナル | Sci Rep |
| Abstract |
Mitotic progression requires the precise formation of spindle microtubules based on mature centrosomes. During the G2/M transition, centrosome maturation progresses, and associated microtubules bundle to form mitotic spindle fibers and capture the chromosomes for alignment at the cell equator. Mitotic kinases-induced phosphorylation signaling is necessary for these processes. Here, we identified SH2 domain-containing protein 4A (SH2D4A/PPP1R38) as a new mitotic regulator. SH2D4A knockdown delays mitotic progression. The time-lapse imaging analysis showed that SH2D4A specifically contributes to the alignment of chromosomes. The cold treatment assay and microtubule regrowth assay indicated that SH2D4A promotes microtubule nucleation to support kinetochore-microtubule attachment. This may be due to the centrosome maturation by SH2D4A via centrosomal recruitment of pericentriolar material (PCM) such as cep192, γ-tubulin, and PLK1. SH2D4A was found to be a negative regulator of PP1 phosphatase. Consistently, treatment with a PP1 inhibitor rescues SH2D4A-knockdown-induced phenotypes, including the microtubule nucleation and centrosomal recruitment of active PLK1. These results suggest that SH2D4A is involved in PCM recruitment to centrosomes and centrosome maturation through attenuation of PP1 phosphatases, accelerating the spindle formation and supporting mitotic progression. |
| 巻・号 | 13(1) |
| ページ | 2067 |
| 公開日 | 2023-2-4 |
| DOI | 10.1038/s41598-023-29362-w |
| PII | 10.1038/s41598-023-29362-w |
| PMID | 36739326 |
| PMC | PMC9899277 |
| MeSH | Centrosome* / metabolism Chromosomal Proteins, Non-Histone / metabolism Humans Intracellular Signaling Peptides and Proteins / metabolism Microtubules / metabolism Mitosis* Spindle Apparatus / metabolism Tubulin / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |