| RRC ID |
75312
|
| Author |
Chaplin AV, Shcherbakova VA, Pikina AP, Sokolova SR, Korzhanova M, Belova VA, Korostin DO, Rebrikov DV, Kardonsky DA, Urban AS, Zakharzhevskaya NB, Suzina NE, Podoprigora IV, Das MS, Kholopova DO, Efimov BA.
|
| Title |
Diplocloster agilis gen. nov., sp. nov. and Diplocloster modestus sp. nov., two novel anaerobic fermentative members of Lachnospiraceae isolated from human faeces.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Three novel strains of Gram-stain-negative, obligately anaerobic, spore-forming straight or slightly curved rods with pointed ends occurring singly or in pairs were isolated from the faeces of healthy human children. The strains were characterized by mesophilic fermentative metabolism and production of acetate, ethanol and H2 as the end metabolic products. Strains ASD3451 and ASD5720T were motile, fermented lactose and raffinose, and weakly fermented maltose. Strain ASD4241T was non-motile and did not ferment the carbohydrates listed above but fermented starch. Strains ASD3451 and ASD5720T shared average nucleotide identity higher than 98.5 % with each other, while ASD4241T had only 88.5-89 % identity to them. Based on phylogenetic and chemotaxonomic analyses, we propose Diplocloster agilis gen. nov., sp. nov. (ASD5720T=JCM 34353T=VKM B-3497T) and Diplocloster modestus sp. nov. (ASD4241T=JCM 34351T=VKM B-3498T) within the family Lachnospiraceae.
|
| Volume |
72(2)
|
| Published |
2022-2-1
|
| DOI |
10.1099/ijsem.0.005222
|
| PMID |
35133260
|
| MeSH |
Anaerobiosis
Bacterial Typing Techniques
Base Composition
Child
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology*
Firmicutes / classification*
Firmicutes / isolation & purification
Humans
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM 34350
JCM 34353
JCM 34351 |
