RRC ID 75796
Author Takahashi C, Harada Y, Ariga H, Iguchi-Ariga SM.
Title Involvement of PIM-1 in DNA fragmentation in mouse NS-1-derived cells.
Journal Biochem Biophys Res Commun
Abstract In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15-20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA ('ladder'). Among a variety of genes so far reported to be related to apoptosis, only Pim-1 was expressed at an elevated level in the NS-1 hybridomas as compared in a control myeloma cell line without 'ladder'. Transfection of a Pim-1 expression vector to a 'ladder'-non-producing myeloma line yielded similar internucleosomal DNA fragmentation. The results hence suggested that Pim-1 activates endonucleases responsible for DNA fragmentation during apoptosis and/or repress DNA repair systems to restore fragmented DNA.
Volume 215(2)
Pages 538-46
Published 1995-10-13
DOI 10.1006/bbrc.1995.2498
PII S0006291X85724989
PMID 7487989
MeSH Animals Apoptosis Base Sequence Cell Line Clone Cells DNA Damage DNA Primers DNA Probes DNA, Neoplasm / isolation & purification DNA, Neoplasm / metabolism* Flow Cytometry Gene Expression Hybridomas Mice Molecular Sequence Data Multiple Myeloma Plasmids Polymerase Chain Reaction Protein Serine-Threonine Kinases / metabolism* Proto-Oncogene Proteins / biosynthesis Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-pim-1 Tumor Cells, Cultured
IF 2.985
Human and Animal Cells Ab1400(RCB0026)