論文 - 詳細
RRC ID | 75796 |
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著者 | Takahashi C, Harada Y, Ariga H, Iguchi-Ariga SM. |
タイトル | Involvement of PIM-1 in DNA fragmentation in mouse NS-1-derived cells. |
ジャーナル | Biochem Biophys Res Commun |
Abstract |
In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15-20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA ('ladder'). Among a variety of genes so far reported to be related to apoptosis, only Pim-1 was expressed at an elevated level in the NS-1 hybridomas as compared in a control myeloma cell line without 'ladder'. Transfection of a Pim-1 expression vector to a 'ladder'-non-producing myeloma line yielded similar internucleosomal DNA fragmentation. The results hence suggested that Pim-1 activates endonucleases responsible for DNA fragmentation during apoptosis and/or repress DNA repair systems to restore fragmented DNA. |
巻・号 | 215(2) |
ページ | 538-46 |
公開日 | 1995-10-13 |
DOI | 10.1006/bbrc.1995.2498 |
PII | S0006291X85724989 |
PMID | 7487989 |
MeSH | Animals Apoptosis Base Sequence Cell Line Clone Cells DNA Damage DNA Primers DNA Probes DNA, Neoplasm / isolation & purification DNA, Neoplasm / metabolism* Flow Cytometry Gene Expression Hybridomas Mice Molecular Sequence Data Multiple Myeloma Plasmids Polymerase Chain Reaction Protein Serine-Threonine Kinases / metabolism* Proto-Oncogene Proteins / biosynthesis Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-pim-1 Tumor Cells, Cultured |
IF | 2.985 |
リソース情報 | |
ヒト・動物細胞 | Ab1400(RCB0026) |