| RRC ID |
75796
|
| Author |
Takahashi C, Harada Y, Ariga H, Iguchi-Ariga SM.
|
| Title |
Involvement of PIM-1 in DNA fragmentation in mouse NS-1-derived cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15-20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA ('ladder'). Among a variety of genes so far reported to be related to apoptosis, only Pim-1 was expressed at an elevated level in the NS-1 hybridomas as compared in a control myeloma cell line without 'ladder'. Transfection of a Pim-1 expression vector to a 'ladder'-non-producing myeloma line yielded similar internucleosomal DNA fragmentation. The results hence suggested that Pim-1 activates endonucleases responsible for DNA fragmentation during apoptosis and/or repress DNA repair systems to restore fragmented DNA.
|
| Volume |
215(2)
|
| Pages |
538-46
|
| Published |
1995-10-13
|
| DOI |
10.1006/bbrc.1995.2498
|
| PII |
S0006291X85724989
|
| PMID |
7487989
|
| MeSH |
Animals
Apoptosis
Base Sequence
Cell Line
Clone Cells
DNA Damage
DNA Primers
DNA Probes
DNA, Neoplasm / isolation & purification
DNA, Neoplasm / metabolism*
Flow Cytometry
Gene Expression
Hybridomas
Mice
Molecular Sequence Data
Multiple Myeloma
Plasmids
Polymerase Chain Reaction
Protein Serine-Threonine Kinases / metabolism*
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-pim-1
Tumor Cells, Cultured
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
Ab1400(RCB0026) |
