RRC ID 75861
Author Trabasso P, Matsuzawa T, Arai T, Hagiwara D, Mikami Y, Moretti ML, Watanabe A.
Title Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene.
Journal Sci Rep
Abstract Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets. It also differentiated strains with TR46 tandem repeats from those with TR34 tandem repeats. These results showed this TR46-LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR46 tandem repeats.
Volume 11(1)
Pages 17087
Published 2021-8-24
DOI 10.1038/s41598-021-96651-7
PII 10.1038/s41598-021-96651-7
PMID 34429488
PMC PMC8384855
MeSH Antifungal Agents / toxicity Aspergillus fumigatus / drug effects Aspergillus fumigatus / genetics* Azoles / toxicity Cytochrome P-450 Enzyme System / genetics* DNA Primers / chemistry DNA Primers / genetics Drug Resistance, Fungal* Fungal Proteins / genetics* Molecular Diagnostic Techniques / methods* Nucleic Acid Amplification Techniques / methods* Promoter Regions, Genetic Tandem Repeat Sequences
IF 3.998
Pathogenic eukaryotic microorganisms Aspergillus fumigatus IFM62918, IFM63432, IFM64460, IFM64733