論文 - 詳細
| RRC ID | 75936 |
|---|---|
| 著者 | De-Castro ARG, Rodrigues DRM, De-Castro MJG, Vieira N, Vieira C, Carvalho AX, Gassmann R, Abreu CMC, Dantas TJ. |
| タイトル | WDR60-mediated dynein-2 loading into cilia powers retrograde IFT and transition zone crossing. |
| ジャーナル | J Cell Biol |
| Abstract |
The dynein-2 motor complex drives retrograde intraflagellar transport (IFT), playing a pivotal role in the assembly and functions of cilia. However, the mechanisms that regulate dynein-2 motility remain poorly understood. Here, we identify the Caenorhabditis elegans WDR60 homologue, WDR-60, and dissect the roles of this intermediate chain using genome editing and live imaging of endogenous dynein-2/IFT components. We find that loss of WDR-60 impairs dynein-2 recruitment to cilia and its incorporation onto anterograde IFT trains, reducing retrograde motor availability at the ciliary tip. Consistent with this, we show that fewer dynein-2 motors power WDR-60-deficient retrograde IFT trains, which move at reduced velocities and fail to exit cilia, accumulating on the distal side of the transition zone. Remarkably, disrupting the transition zone's NPHP module almost fully restores ciliary exit of underpowered retrograde trains in wdr-60 mutants. This work establishes WDR-60 as a major contributor to IFT, and the NPHP module as a roadblock to dynein-2 passage through the transition zone. |
| 巻・号 | 221(1) |
| 公開日 | 2022-1-3 |
| DOI | 10.1083/jcb.202010178 |
| PII | 212746 |
| PMID | 34739033 |
| PMC | PMC8576871 |
| MeSH | Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Cilia / metabolism* Cytoskeletal Proteins / chemistry Cytoskeletal Proteins / metabolism* Dyneins / chemistry Dyneins / metabolism* Flagella / metabolism* Green Fluorescent Proteins / metabolism Kinetics Mutation / genetics Protein Domains Sensory Receptor Cells / metabolism |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 72 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6453 tm3100 tm2452 tm4927 tm925 |