Reference - Detail
| RRC ID | 75944 |
|---|---|
| Author | Dietz S, Almeida MV, Nischwitz E, Schreier J, Viceconte N, Fradera-Sola A, Renz C, Ceron-Noriega A, Ulrich HD, Kappei D, Ketting RF, Butter F. |
| Title | The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1. |
| Journal | Nat Commun |
| Abstract |
Telomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approach to identify TEBP-1 and TEBP-2, two paralogs expressed in the germline and embryogenesis that associate to telomeres in vitro and in vivo. tebp-1 and tebp-2 mutants display strikingly distinct phenotypes: tebp-1 mutants have longer telomeres than wild-type animals, while tebp-2 mutants display shorter telomeres and a Mortal Germline. Notably, tebp-1;tebp-2 double mutant animals have synthetic sterility, with germlines showing signs of severe mitotic and meiotic arrest. Furthermore, we show that POT-1 forms a telomeric complex with TEBP-1 and TEBP-2, which bridges TEBP-1/-2 with POT-2/MRT-1. These results provide insights into the composition and organization of a telomeric protein complex in C. elegans. |
| Volume | 12(1) |
| Pages | 2668 |
| Published | 2021-5-11 |
| DOI | 10.1038/s41467-021-22861-2 |
| PII | 10.1038/s41467-021-22861-2 |
| PMID | 33976151 |
| PMC | PMC8113555 |
| MeSH | Animals Animals, Genetically Modified Base Sequence Binding Sites / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / classification Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA / genetics DNA / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Germ Cells / metabolism Microscopy, Fluorescence / methods Multiprotein Complexes / genetics Multiprotein Complexes / metabolism* Mutation Phylogeny Protein Binding Protein Isoforms / classification Protein Isoforms / genetics Protein Isoforms / metabolism Telomere / genetics Telomere / metabolism* Telomere-Binding Proteins / classification Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism* |
| Resource | |
| C.elegans | tm1400 tm1354 tm1620 |