RRC ID 75944
Author Dietz S, Almeida MV, Nischwitz E, Schreier J, Viceconte N, Fradera-Sola A, Renz C, Ceron-Noriega A, Ulrich HD, Kappei D, Ketting RF, Butter F.
Title The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1.
Journal Nat Commun
Abstract Telomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approach to identify TEBP-1 and TEBP-2, two paralogs expressed in the germline and embryogenesis that associate to telomeres in vitro and in vivo. tebp-1 and tebp-2 mutants display strikingly distinct phenotypes: tebp-1 mutants have longer telomeres than wild-type animals, while tebp-2 mutants display shorter telomeres and a Mortal Germline. Notably, tebp-1;tebp-2 double mutant animals have synthetic sterility, with germlines showing signs of severe mitotic and meiotic arrest. Furthermore, we show that POT-1 forms a telomeric complex with TEBP-1 and TEBP-2, which bridges TEBP-1/-2 with POT-2/MRT-1. These results provide insights into the composition and organization of a telomeric protein complex in C. elegans.
Volume 12(1)
Pages 2668
Published 2021-5-11
DOI 10.1038/s41467-021-22861-2
PII 10.1038/s41467-021-22861-2
PMID 33976151
PMC PMC8113555
MeSH Animals Animals, Genetically Modified Base Sequence Binding Sites / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / classification Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA / genetics DNA / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Germ Cells / metabolism Microscopy, Fluorescence / methods Multiprotein Complexes / genetics Multiprotein Complexes / metabolism* Mutation Phylogeny Protein Binding Protein Isoforms / classification Protein Isoforms / genetics Protein Isoforms / metabolism Telomere / genetics Telomere / metabolism* Telomere-Binding Proteins / classification Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism*
C.elegans tm1400 tm1354 tm1620