論文 - 詳細
| RRC ID | 75976 |
|---|---|
| 著者 | Huang X, Cheng P, Weng C, Xu Z, Zeng C, Xu Z, Chen X, Zhu C, Guang S, Feng X. |
| タイトル | A chromodomain protein mediates heterochromatin-directed piRNA expression. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
PIWI-interacting RNAs (piRNAs) play significant roles in suppressing transposons, maintaining genome integrity, and defending against viral infections. How piRNA source loci are efficiently transcribed is poorly understood. Here, we show that in Caenorhabditis elegans, transcription of piRNA clusters depends on the chromatin microenvironment and a chromodomain-containing protein, UAD-2. piRNA clusters form distinct focus in germline nuclei. We conducted a forward genetic screening and identified UAD-2 that is required for piRNA focus formation. In the absence of histone 3 lysine 27 methylation or proper chromatin-remodeling status, UAD-2 is depleted from the piRNA focus. UAD-2 recruits the upstream sequence transcription complex (USTC), which binds the Ruby motif to piRNA promoters and promotes piRNA generation. Vice versa, the USTC complex is required for UAD-2 to associate with the piRNA focus. Thus, transcription of heterochromatic small RNA source loci relies on coordinated recruitment of both the readers of histone marks and the core transcriptional machinery to DNA. |
| 巻・号 | 118(27) |
| 公開日 | 2021-7-6 |
| DOI | 10.1073/pnas.2103723118 |
| PII | 2103723118 |
| PMID | 34187893 |
| PMC | PMC8271797 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Chromatin Assembly and Disassembly Genetic Testing Germ Cells / cytology Germ Cells / metabolism Heterochromatin / metabolism* Histones / metabolism Lysine / metabolism Methylation Peptides / metabolism Polycomb Repressive Complex 2 / metabolism Protein Binding RNA, Small Interfering / metabolism* Temperature |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6157 tm1227 |