RRC ID 75987
Author Kim H, Ding YH, Lu S, Zuo MQ, Tan W, Conte D Jr, Dong MQ, Mello CC.
Title PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans.
Journal Elife
Abstract Germlines shape and balance heredity, integrating and regulating information from both parental and foreign sources. Insights into how germlines handle information have come from the study of factors that specify or maintain the germline fate. In early Caenorhabditis elegans embryos, the CCCH zinc finger protein PIE-1 localizes to the germline where it prevents somatic differentiation programs. Here, we show that PIE-1 also functions in the meiotic ovary where it becomes SUMOylated and engages the small ubiquitin-like modifier (SUMO)-conjugating machinery. Using whole-SUMO-proteome mass spectrometry, we identify HDAC SUMOylation as a target of PIE-1. Our analyses of genetic interactions between pie-1 and SUMO pathway mutants suggest that PIE-1 engages the SUMO machinery both to preserve the germline fate in the embryo and to promote Argonaute-mediated surveillance in the adult germline.
Volume 10
Published 2021-5-18
DOI 10.7554/eLife.63300
PII 63300
PMID 34003111
PMC PMC8131105
MeSH Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Cell Differentiation Female Meiosis Nuclear Proteins / genetics* Nuclear Proteins / metabolism* Ovum RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Sumoylation / genetics*
Resource
C.elegans tm2723