論文 - 詳細
| RRC ID | 76093 |
|---|---|
| 著者 | Xia SL, Li M, Chen B, Wang C, Yan YH, Dong MQ, Qi YB. |
| タイトル | The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling. |
| ジャーナル | Elife |
| Abstract |
Neural circuits develop through a plastic phase orchestrated by genetic programs and environmental signals. We have identified a leucine-rich-repeat domain transmembrane protein PAN-1 as a factor required for synaptic rewiring in C. elegans. PAN-1 localizes on cell membrane and binds with MYRF, a membrane-bound transcription factor indispensable for promoting synaptic rewiring. Full-length MYRF was known to undergo self-cleavage on ER membrane and release its transcriptional N-terminal fragment in cultured cells. We surprisingly find that MYRF trafficking to cell membrane before cleavage is pivotal for C. elegans development and the timing of N-MYRF release coincides with the onset of synaptic rewiring. On cell membrane PAN-1 and MYRF interact with each other via their extracellular regions. Loss of PAN-1 abolishes MYRF cell membrane localization, consequently blocking myrf-dependent neuronal rewiring process. Thus, through interactions with a cooperating factor on the cell membrane, MYRF may link cell surface activities to transcriptional cascades required for development. |
| 巻・号 | 10 |
| 公開日 | 2021-5-5 |
| DOI | 10.7554/eLife.67628 |
| PII | 67628 |
| PMID | 33950834 |
| PMC | PMC8099431 |
| MeSH | Animals CRISPR-Cas Systems Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Membrane / metabolism HEK293 Cells Humans Protein Domains Protein Transport Synapses / physiology Transcription Factors / genetics Transcription Factors / metabolism* |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tmIs1073 tmIs1072 |