RRC ID |
76093
|
Author |
Xia SL, Li M, Chen B, Wang C, Yan YH, Dong MQ, Qi YB.
|
Title |
The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling.
|
Journal |
Elife
|
Abstract |
Neural circuits develop through a plastic phase orchestrated by genetic programs and environmental signals. We have identified a leucine-rich-repeat domain transmembrane protein PAN-1 as a factor required for synaptic rewiring in C. elegans. PAN-1 localizes on cell membrane and binds with MYRF, a membrane-bound transcription factor indispensable for promoting synaptic rewiring. Full-length MYRF was known to undergo self-cleavage on ER membrane and release its transcriptional N-terminal fragment in cultured cells. We surprisingly find that MYRF trafficking to cell membrane before cleavage is pivotal for C. elegans development and the timing of N-MYRF release coincides with the onset of synaptic rewiring. On cell membrane PAN-1 and MYRF interact with each other via their extracellular regions. Loss of PAN-1 abolishes MYRF cell membrane localization, consequently blocking myrf-dependent neuronal rewiring process. Thus, through interactions with a cooperating factor on the cell membrane, MYRF may link cell surface activities to transcriptional cascades required for development.
|
Volume |
10
|
Published |
2021-5-5
|
DOI |
10.7554/eLife.67628
|
PII |
67628
|
PMID |
33950834
|
PMC |
PMC8099431
|
MeSH |
Animals
CRISPR-Cas Systems
Caenorhabditis elegans / chemistry*
Caenorhabditis elegans / genetics
Caenorhabditis elegans / physiology
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Membrane / metabolism
HEK293 Cells
Humans
Protein Domains
Protein Transport
Synapses / physiology
Transcription Factors / genetics
Transcription Factors / metabolism*
|
Resource |
C.elegans |
tmIs1073
tmIs1072 |