RRC ID 76093
Author Xia SL, Li M, Chen B, Wang C, Yan YH, Dong MQ, Qi YB.
Title The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling.
Journal Elife
Abstract Neural circuits develop through a plastic phase orchestrated by genetic programs and environmental signals. We have identified a leucine-rich-repeat domain transmembrane protein PAN-1 as a factor required for synaptic rewiring in C. elegans. PAN-1 localizes on cell membrane and binds with MYRF, a membrane-bound transcription factor indispensable for promoting synaptic rewiring. Full-length MYRF was known to undergo self-cleavage on ER membrane and release its transcriptional N-terminal fragment in cultured cells. We surprisingly find that MYRF trafficking to cell membrane before cleavage is pivotal for C. elegans development and the timing of N-MYRF release coincides with the onset of synaptic rewiring. On cell membrane PAN-1 and MYRF interact with each other via their extracellular regions. Loss of PAN-1 abolishes MYRF cell membrane localization, consequently blocking myrf-dependent neuronal rewiring process. Thus, through interactions with a cooperating factor on the cell membrane, MYRF may link cell surface activities to transcriptional cascades required for development.
Volume 10
Published 2021-5-5
DOI 10.7554/eLife.67628
PII 67628
PMID 33950834
PMC PMC8099431
MeSH Animals CRISPR-Cas Systems Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Membrane / metabolism HEK293 Cells Humans Protein Domains Protein Transport Synapses / physiology Transcription Factors / genetics Transcription Factors / metabolism*
Resource
C.elegans tmIs1073 tmIs1072