| RRC ID |
76151
|
| 著者 |
Dai S, Tang X, Li L, Ishidate T, Ozturk AR, Chen H, Dude AL, Yan YH, Dong MQ, Shen EZ, Mello CC.
|
| タイトル |
A family of C. elegans VASA homologs control Argonaute pathway specificity and promote transgenerational silencing.
|
| ジャーナル |
Cell Rep
|
| Abstract |
Germline Argonautes direct transcriptome surveillance within perinuclear membraneless organelles called nuage. In C. elegans, a family of Vasa-related Germ Line Helicase (GLH) proteins localize in and promote the formation of nuage. Previous studies have implicated GLH proteins in inherited silencing, but direct roles in small-RNA production, Argonaute binding, or mRNA targeting have not been identified. Here we show that GLH proteins compete with each other to control Argonaute pathway specificity, bind directly to Argonaute target mRNAs, and promote the amplification of small RNAs required for transgenerational inheritance. We show that the ATPase cycle of GLH-1 regulates direct binding to the Argonaute WAGO-1, which engages amplified small RNAs. Our findings support a dynamic and direct role for GLH proteins in inherited silencing beyond their role as structural components of nuage.
|
| 巻・号 |
40(10)
|
| ページ |
111265
|
| 公開日 |
2022-9-6
|
| DOI |
10.1016/j.celrep.2022.111265
|
| PII |
S2211-1247(22)01083-X
|
| PMID |
36070689
|
| PMC |
PMC9887883
|
| MeSH |
Animals
Argonaute Proteins / genetics
Argonaute Proteins / metabolism
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / metabolism
DEAD-box RNA Helicases / metabolism
Germ Cells / metabolism
RNA, Messenger / metabolism
|
| リソース情報 |
| 線虫 |
tm872 |
